Immobilization of papain on chitin and chitosan and recycling of soluble enzyme for deflocculation of saccharomyces cerevisiae from bioethanol distilleries

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dc.contributor.authorSilva, Douglas Fernandes [UNESP]
dc.contributor.authorRosa, Henrique [UNESP]
dc.contributor.authorCarvalho, Ana Flavia Azevedo
dc.contributor.authorOliva Neto, Pedro de [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2015-08-21T17:54:00Z
dc.date.available2015-08-21T17:54:00Z
dc.date.issued2015
dc.description.abstractYeast flocculation (Saccharomyces cerevisiae) is one of the most important problems in fuel ethanol production. Yeast flocculation causes operational difficulties and increase in the ethanol cost. Proteolytic enzymes can solve this problem since it does not depend on these changes. The recycling of soluble papain and the immobilization of this enzyme on chitin or chitosan were studied. Some cross-linking agents were evaluated in the action of proteolytic activity of papain. The glutaraldehyde (0.1–10%?w·v-1), polyethyleneimine (0.5%?v·v-1), and tripolyphosphate (1–10%?w·v-1) inactivated the enzyme in this range, respectively. Glutaraldehyde inhibited all treatments of papain immobilization. The chitosan cross-linked with TPP in 5?h of reaction showed the yield of active immobilized enzyme of 15.7% and 6.07% in chitosan treated with 0.1% PEI. Although these immobilizations have been possible, these levels have not been enough to cause deflocculation of yeast cells. Free enzyme was efficient for yeast deflocculation in dosages of 3 to 4?g·L-1. Recycling of soluble papain by centrifugation was effective for 14 cycles with yeast suspension in time perfectly compatible to industrial conditions. The reuse of proteases applied after yeast suspension by additional yeast centrifugation could be an alternative to cost reduction of these enzymes.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Psicologia Clínica, Faculdade de Ciências e Letras de Assis, Assis, DEPT.CIENCIAS BIOLOGICAS - FAC.CIENCIAS E LETRAS DE ASSIS - UNESP, P.UNIVERSITARIO, CEP 19800-000, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Psicologia Clínica, Faculdade de Ciências e Letras de Assis, Assis, DEPT.CIENCIAS BIOLOGICAS - FAC.CIENCIAS E LETRAS DE ASSIS - UNESP, P.UNIVERSITARIO, CEP 19800-000, SP, Brasil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent1-10
dc.identifierhttp://www.hindawi.com/journals/er/2015/573721/
dc.identifier.citationEnzyme Research, v. 2015, p. 1-10, 2015.
dc.identifier.doi10.1155/2015/573721
dc.identifier.fileISSN2090-0406-2015-2015-01-10.pdf
dc.identifier.issn2090-0406
dc.identifier.lattes4638952263502744
dc.identifier.orcid0000-0001-9378-9036
dc.identifier.urihttp://hdl.handle.net/11449/127146
dc.language.isoeng
dc.relation.ispartofEnzyme Research
dc.relation.ispartofsjr0,653
dc.rights.accessRightsAcesso aberto
dc.sourceCurrículo Lattes
dc.subjectDesfloculaçãoen
dc.subjectlEvedurasen
dc.subjectImobilizaçãoen
dc.subjectPapaínaen
dc.titleImmobilization of papain on chitin and chitosan and recycling of soluble enzyme for deflocculation of saccharomyces cerevisiae from bioethanol distilleriesen
dc.typeArtigo
unesp.author.lattes4638952263502744[4]
unesp.author.orcid0000-0001-9378-9036[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências e Letras, Assispt
unesp.departmentPsicologia Clínicapt

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Artigos - Ciências Biológicas - FCLAS