Metalloproteomic profile determination of muscle samples from nile Tilapia (Oreochromis niloticus) using AAS and ESI-MS/MS after 2D-PAGE separation

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dc.contributor.authorCavecci, Bruna [UNESP]
dc.contributor.authorLima, Paula M. de [UNESP]
dc.contributor.authorQueiroz, Joao V. de [UNESP]
dc.contributor.authorBraga, Camila P. [UNESP]
dc.contributor.authorPadilha, Cilene C. F. [UNESP]
dc.contributor.authorLeite, Aline L. [UNESP]
dc.contributor.authorBuzalaf, Marilia A. R. [UNESP]
dc.contributor.authorPezzato, Luiz E. [UNESP]
dc.contributor.authorPadilha, Pedro M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-10-21T13:11:57Z
dc.date.available2015-10-21T13:11:57Z
dc.date.issued2015-02-01
dc.description.abstractThis study evaluated the metalloproteomic profile of muscle tissue samples from Nile tilapia (Oreochromis niloticus). Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) methods was used for protein fractionation and identification based on image analysis. Determination of Ca, Cu, Fe, Mn and Zn was performed in the protein spots by flame and/or graphite furnace atomic absorption spectrometry (FAAS, GFAAS), the protein spots were characterized by electrospray ionization-tandem mass spectrometry (ESI-MS/MS). FAAS and GFAAS determinations have indicated the presence of calcium in seven protein spots, iron in only one spot, manganese in six spots, and zinc in two spots. Of the seventeen protein spots where the presence of metal ions was detected, ten were characterized by ESI-MS/MS.en
dc.description.affiliationUNESP, Coll Vet &Anim Sci, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUNESP, Inst Biosci, Dept Chem &Biochem, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUNESP, Dept Biol Sci, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Coll Vet &Anim Sci, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Biosci, Dept Chem &Biochem, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Biol Sci, BR-18618970 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2011/14902-0
dc.description.sponsorshipIdFAPESP: 2010/51332-5
dc.format.extent239-246
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015000200239&lng=en&nrm=iso&tlng=en
dc.identifier.citationJournal Of The Brazilian Chemical Society, v. 26, n. 2, p. 239-246, 2015.
dc.identifier.doi10.5935/0103-5053.20140260
dc.identifier.fileS0103-50532015000200239.pdf
dc.identifier.issn0103-5053
dc.identifier.lattes6981448637456391
dc.identifier.orcid0000-0003-4179-0574
dc.identifier.scieloS0103-50532015000200239
dc.identifier.urihttp://hdl.handle.net/11449/128655
dc.identifier.wosWOS:000351850100005
dc.language.isoeng
dc.publisherSoc Brasileira Quimica
dc.relation.ispartofJournal Of The Brazilian Chemical Society
dc.relation.ispartofjcr1.444
dc.relation.ispartofsjr0,357
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectMetalloproteinsen
dc.subjectESI-MS/MSen
dc.subject2D-PAGEen
dc.titleMetalloproteomic profile determination of muscle samples from nile Tilapia (Oreochromis niloticus) using AAS and ESI-MS/MS after 2D-PAGE separationen
dc.typeArtigo
dcterms.rightsHolderSoc Brasileira Quimica
unesp.author.lattes6981448637456391[9]
unesp.author.orcid0000-0003-4179-0574[9]
unesp.author.orcid0000-0001-5447-8913[1]
unesp.author.orcid0000-0002-2112-1309[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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