Improvement of lipase obtaining system by orange waste-based solid-state fermentation: production, characterization and application

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dc.contributor.authorAthanázio-Heliodoro, Julia Carolina [UNESP]
dc.contributor.authorOkino-Delgado, Clarissa Hamaio [UNESP]
dc.contributor.authorFernandes, Célio Júnior da Costa [UNESP]
dc.contributor.authorZanutto, Mirella Rossitto [UNESP]
dc.contributor.authorPrado, Débora Zanoni do [UNESP]
dc.contributor.authorda Silva, Rodrigo Augusto [UNESP]
dc.contributor.authorFacanali, Roselaine
dc.contributor.authorZambuzzi, Willian Fernando [UNESP]
dc.contributor.authorMarques, Márcia Ortiz Mayo
dc.contributor.authorFleuri, Luciana Francisco [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionAgronomic Institute (IAC)
dc.date.accessioned2019-10-06T17:11:40Z
dc.date.available2019-10-06T17:11:40Z
dc.date.issued2018-08-09
dc.description.abstractLipases are an economic important group of biocatalysts that can be produced by some fungal under solid-state fermentation. Orange wastes are source of lipases and potential substrates for lipases production. This work assessed 19 fugal strains cultivated in Citrus sinensis cv. Hamlin orange wastes (peel, frit and core) for production of lipases in order to generate compounds with antioxidant, antimicrobial and cytotoxic properties. Fifteen of those fungi grew and produced lipases, mainly the Aspergillus brasiliensis [National Institute of Quality Control (INCQS) 40036]/frit system, which showed 99.58 U/g total lipase. The substrate with the highest production of lipase was frit with 26.67 and 78.91 U/g of total lipases produced on average by the 15 microorganisms. Aspergillus niger 01/frit (33.53 U/g) and Aspergillus niger (INCQS 40015)/frit (34.76 U/g) systems showed the highest specificity values in all the herein tested synthetic substrates with 4, 12 and 16 carbons. Analysis of the fatty acid profile of hydrolysis products obtained in the most prominent systems applied to corn and sunflower oils showed: palmitic acid, linoleic acid, oleic acid, and stearic acid. These acids showed antioxidant capacity of up to 58% DPPH (2,2-diphenyl-1-pierylhydrazyl) radical reduction and antibacterial activity against Escherichia coli, Listeria monocytogenes, Pseudomonas aureginosa, Salmonella Enteritidis and Staphylococcus aureus, as well as cytotoxicity to SCC9 cells (squamous cancer cells).en
dc.description.affiliationChemistry and Biochemistry Department Institute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationAgronomic Institute (IAC)
dc.description.affiliationUnespChemistry and Biochemistry Department Institute of Biosciences São Paulo State University (UNESP)
dc.format.extent565-573
dc.identifierhttp://dx.doi.org/10.1080/10826068.2018.1476879
dc.identifier.citationPreparative Biochemistry and Biotechnology, v. 48, n. 7, p. 565-573, 2018.
dc.identifier.doi10.1080/10826068.2018.1476879
dc.identifier.issn1532-2297
dc.identifier.issn1082-6068
dc.identifier.lattes6495148747049688
dc.identifier.scopus2-s2.0-85067231678
dc.identifier.urihttp://hdl.handle.net/11449/190392
dc.language.isoeng
dc.relation.ispartofPreparative Biochemistry and Biotechnology
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAntioxidant
dc.subjectfrit
dc.subjectHamlin
dc.subjectlipase
dc.subjectmicrobial
dc.subjectorange
dc.titleImprovement of lipase obtaining system by orange waste-based solid-state fermentation: production, characterization and applicationen
dc.typeResenha
unesp.author.lattes6495148747049688
unesp.author.orcid0000-0002-8648-9550[1]
unesp.author.orcid0000-0002-0438-2478[10]

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