Alterations of protein expression in conditions of copper-deprivation for Paracoccidioides lutzii in the presence of extracellular matrix components

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dc.contributor.authorOliveira, Haroldo Cesar de [UNESP]
dc.contributor.authorSilva, Julhiany de Fatima da [UNESP]
dc.contributor.authorMatsumoto, Marcelo Teruyuki [UNESP]
dc.contributor.authorMarcos, Caroline Maria [UNESP]
dc.contributor.authorSilva, Roberta Peres da [UNESP]
dc.contributor.authorMoraes da Silva, Rosangela Aparecida [UNESP]
dc.contributor.authorVeneziano Labate, Monica Teresa
dc.contributor.authorLabate, Carlos Alberto
dc.contributor.authorFusco Almeida, Ana Marisa [UNESP]
dc.contributor.authorMendes-Giannini, Maria José Soares [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2015-11-03T15:29:43Z
dc.date.available2015-11-03T15:29:43Z
dc.date.issued2014-12-13
dc.description.abstractBackground: Paracoccidioides spp is a fungi genus and the agent of paracoccidioidomycosis. The strategies of infection used by these pathogens involve the expression of proteins related to adaptation to the host, particularly regarding the uptake of micronutrients. This study analyzed the adhesion of Paracoccidioides lutzii during conditions of copper (Cu) and iron (Fe) deprivation, while also evaluating the proteins expressed in conditions of Cu depletion in the presence of four extracellular matrix (ECM) components (laminin, fibronectin and types I and IV collagen).Results: We cultured the P. lutzii in a chemically defined media without Cu and Fe. The fungus was then placed in contact with different ECM components and adhesion was evaluated. A significant increase in binding to all ECM components was observed when the fungus was cultured without Cu; which might be related to some adhesins expression. A proteomic assay was developed and revealed 39 proteins expressed that are involved in processes such as virulence, protein synthesis, metabolism, energy, transcription, transport, stress response and the cell cycle when the fungus was interacting with the ECM components. The up-regulated expression of two important adhesins, enolase and 14-3-3, was observed at the fungal cell wall during the interaction with the ECM components, indicating the role of these proteins in the Paracoccidioides-host interaction.Conclusions: This study is important for determining prospective proteins that may be involved in the interaction of Paracoccidioides with a host. Understanding the adaptive response to different growth conditions, elucidating the processes of adhesion and cell invasion, and identifying the proteins that are differentially expressed during the fungus-host interaction may help elucidate mechanisms used for survival and growth of Paracoccidioides in various human tissues.en
dc.description.affiliationUniv Estadual Paulista, UNESP, Lab Micol Clin, Fac Ciencias Farmaceut,Dept Anal Clin, Araraquara, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, Lab Multiusuarios Ctr Genom Func Aplicada Agr &A, Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Lab Micol Clin, Fac Ciencias Farmaceut,Dept Anal Clin, Araraquara, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipNational Council for Scientific and Technological Development
dc.description.sponsorshipPADCFCF-Sao Paulo State University
dc.description.sponsorshipIdFAPESP: 2008/56476-5
dc.description.sponsorshipIdFAPESP: 2009/52046-9
dc.description.sponsorshipIdFAPESP: 2011/18038-9
dc.description.sponsorshipIdNational Council for Scientific and Technological Development: 473119/2010-2
dc.format.extent1-16
dc.identifierhttp://www.biomedcentral.com/1471-2180/14/302
dc.identifier.citationBmc Microbiology. London: Biomed Central Ltd, v. 14, 16 p., 2014.
dc.identifier.doi10.1186/s12866-014-0302-7
dc.identifier.fileWOS000348166400001.pdf
dc.identifier.issn1471-2180
dc.identifier.orcid0000-0002-8059-0826
dc.identifier.urihttp://hdl.handle.net/11449/130155
dc.identifier.wosWOS:000348166400001
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofBmc Microbiology
dc.relation.ispartofjcr2.829
dc.relation.ispartofsjr1,242
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectParacoccidioides sppen
dc.subjectCopperen
dc.subjectAdhesionen
dc.subjectProtein expressionen
dc.subjectParacoccidioidomycosisen
dc.titleAlterations of protein expression in conditions of copper-deprivation for Paracoccidioides lutzii in the presence of extracellular matrix componentsen
dc.typeArtigo
dcterms.rightsHolderBiomed Central Ltd
unesp.author.lattes3716273524139678[9]
unesp.author.orcid0000-0002-8059-0826[10]
unesp.author.orcid0000-0002-2115-8988[9]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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