Protocolo de feminilização em Tilápia Oreochromis niloticus submetidas ao tratamento com hormônio estrógeno 17-β-estradiol
Carregando...
Data
2023-07-14
Autores
Nova, Fabio Augusto Paiva Camisa
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
O presente trabalho teve como objetivo verificar por métodos moleculares e fenotípico, o
protocolo de feminilização de Tilápia Oreochromis niloticus através da utilização do
hormônio estrógeno 17-β-estradiol incluso na ração na fase inicial de produção de
neofêmeas XY. O experimento foi conduzido no Campus Agrochá da FCAVR – UNESP
Registro e no Laboratório de Genética em Aquicultura e Conservação – LaGeAC do
Centro de Aquicultura da UNESP (CAUNESP), onde foram realizadas as análises
moleculares. Inicialmente um lote de animais selecionados foram submetidos ao processo
de feminilização para obtenção das fêmeas XY (neofêmeas), seguindo duas etapas. Na
primeira etapa, larvas recém geradas, ainda indiferenciadas, foram submetidas a dietas
contendo hormônio estrógeno 17-β-estradiol para produzir neofêmeas, posteriormente
foram coletados 105 indivíduos com cerca de 20 g obtidos na 1ª etapa para serem
avaliados por técnicas moleculares usando um marcador específico do cromossoma Y,
por fim foram identificadas nessa segunda etapa as neofêmeas que foram então mantidas
por um tempo para o crescimento até alcançar 200g onde se foi possível verificar as
papilas genitálias e assim verificar quanto o sexo. A validação utilizando o marcador
específico do cromossomo Y revelou que, dos 105 animais avaliados, 21 apresentavam o
genótipo XY em seu código genético e o restante apresentaram o genótipo XX. Na análise
fenotípica avaliamos 11 dos 21 animais que apresentaram o Y, e foram identificados 6
machos e 5 fêmeas dentro do plantel.
The present study aimed to verify by molecular and phenotypic methods, the protocol of feminization of Tilapia Oreochromis niloticus through the use of the estrogen hormone 17-β-estradiol included in the ration in the initial phase of production of XY neofemales. The experiment was conducted at the Agrochá Campus of FCAVR – UNESP Registro and at the Laboratory of Genetics in Aquaculture and Conservation – LaGeAC of the UNESP Aquaculture Center (CAUNESP), where the molecular analyses were performed. Initially, a batch of selected animals were submitted to the feminization process to obtain XY females (neo-females), following two stages. In the first stage, newly generated larvae, still undifferentiated, were submitted to diets containing estrogen hormone 17-β-estradiol to produce neofemales, later 105 individuals with about 20 g obtained in the 1st step were collected to be evaluated by molecular techniques using a specific marker of the Y chromosome. Finally, in this second stage, the neofemales were identified, which were then maintained for a while for growth until reaching 200g, where it was possible to verify the papillae, genitalia and thus verify how much sex. Validation using the specific marker of the Y chromosome revealed that, of the 105 animals evaluated, 21 had the XY genotype in their genetic code and the rest presented the XX genotype. In the phenotypic analysis we evaluated 11 of the 21 animals that presented the Y, and 6 males and 5 females were identified within the herd.
The present study aimed to verify by molecular and phenotypic methods, the protocol of feminization of Tilapia Oreochromis niloticus through the use of the estrogen hormone 17-β-estradiol included in the ration in the initial phase of production of XY neofemales. The experiment was conducted at the Agrochá Campus of FCAVR – UNESP Registro and at the Laboratory of Genetics in Aquaculture and Conservation – LaGeAC of the UNESP Aquaculture Center (CAUNESP), where the molecular analyses were performed. Initially, a batch of selected animals were submitted to the feminization process to obtain XY females (neo-females), following two stages. In the first stage, newly generated larvae, still undifferentiated, were submitted to diets containing estrogen hormone 17-β-estradiol to produce neofemales, later 105 individuals with about 20 g obtained in the 1st step were collected to be evaluated by molecular techniques using a specific marker of the Y chromosome. Finally, in this second stage, the neofemales were identified, which were then maintained for a while for growth until reaching 200g, where it was possible to verify the papillae, genitalia and thus verify how much sex. Validation using the specific marker of the Y chromosome revealed that, of the 105 animals evaluated, 21 had the XY genotype in their genetic code and the rest presented the XX genotype. In the phenotypic analysis we evaluated 11 of the 21 animals that presented the Y, and 6 males and 5 females were identified within the herd.
Descrição
Palavras-chave
População monosexo, Sexo (Biologia), Identificação do sexo, Cromossomo Y, Monosex population, Gender identification, Sex (Biology), Y Chromosome