Clastogenicity of Piper cubeba (Piperaceae) seed extract in an in vivo mammalian cell system

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dc.contributor.authorJunqueira, Adriana Pereira Freire [UNESP]
dc.contributor.authorPerazzo, Fabio Ferreira
dc.contributor.authorSouza, Gustavo Henrique Bianco
dc.contributor.authorMaistro, Edson Luis [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal do Amapá (UNIFAP)
dc.contributor.institutionUniversidade Federal de Ouro Preto (UFOP)
dc.date.accessioned2014-05-20T13:31:41Z
dc.date.available2014-05-20T13:31:41Z
dc.date.issued2007-01-01
dc.description.abstractThe plant Piper cubeba is widely distributed in tropical and subtropical regions and is used medically for various purposes but has not yet been evaluated for genotoxicity. We used male and female Swiss mice and Wistar rats and the comet assay and micronucleus test to investigate the mutagenic potential of a crude extract of P. cubeba seeds. The rodents were administered 0.5 g kg-1, 1.0 g kg-1 and 1.5 g kg-1 of the extract by gavage. For the Swiss mice, peripheral blood was collected 24 h after treatment for the comet assay, and at 48 and 72 h for the micronucleus test. For the Wistar rats, peripheral blood and hepatic cells were collected for the comet assay and bone marrow cells were collected for the micronucleus test 24 h after treatment. At 1.5 g kg-1, the highest dose tested, the extract induced a statistically significant increase in both the mean number of micronucleated polychromatic erythrocytes and the level of DNA damage in the rodent cell types analyzed. Under our experimental conditions, the P. cubeba seed extract was genotoxic in vivo when administered orally to mice and rats.en
dc.description.affiliationUniversidade Estadual Paulista Faculdade de Filosofia e Ciências Departamento de Fonoaudiologia
dc.description.affiliationUniversidade Federal do Amapá Laboratório de Pesquisa em Fármacos
dc.description.affiliationUniversidade Federal de Ouro Preto Departamento de Farmácia Laboratório de Farmacognosia
dc.description.affiliationUnespUniversidade Estadual Paulista Faculdade de Filosofia e Ciências Departamento de Fonoaudiologia
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent656-663
dc.identifierhttp://dx.doi.org/10.1590/S1415-47572007000400025
dc.identifier.citationGenetics and Molecular Biology. Sociedade Brasileira de Genética, v. 30, n. 3, p. 656-663, 2007.
dc.identifier.doi10.1590/S1415-47572007000400025
dc.identifier.fileS1415-47572007000400025.pdf
dc.identifier.issn1415-4757
dc.identifier.lattes4787521613038315
dc.identifier.orcid0000-0003-0757-7876
dc.identifier.scieloS1415-47572007000400025
dc.identifier.urihttp://hdl.handle.net/11449/10796
dc.language.isoeng
dc.publisherSociedade Brasileira de Genética
dc.relation.ispartofGenetics and Molecular Biology
dc.relation.ispartofjcr1.493
dc.relation.ispartofsjr0,638
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectmicronucleus testen
dc.subjectPiper cubebaen
dc.subjectsingle cell gel electrophoresis (SCGE)en
dc.titleClastogenicity of Piper cubeba (Piperaceae) seed extract in an in vivo mammalian cell systemen
dc.typeArtigo
unesp.author.lattes4787521613038315[4]
unesp.author.orcid0000-0003-0757-7876[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Filosofia e Ciências, Maríliapt

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Artigos - Fonoaudiologia - FFC