Physicochemical characterization of nanoparticles formed between DNA and phosphorylcholine substituted chitosans

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dc.contributor.authorCase, Ana Helena [UNESP]
dc.contributor.authorDalla Picola, Isadora Pfeifer [UNESP]
dc.contributor.authorDarbello Zaniquelli, Maria Elisabete
dc.contributor.authorFernandes, Julio Cesar
dc.contributor.authorTaboga, Sebastiao Roberto [UNESP]
dc.contributor.authorWinnik, Francoise M.
dc.contributor.authorTiera, Marcio Jose [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionHop Sacre Coeur
dc.contributor.institutionUniv Montreal
dc.date.accessioned2014-05-20T14:01:05Z
dc.date.available2014-05-20T14:01:05Z
dc.date.issued2009-08-01
dc.description.abstractThe interactions between phosphorylcholine-substituted chitosans (PC-CH) and calf-thymus DNA (ct-DNA) were investigated focusing on the effects of the charge ratio, the pH, and phosphorylcholine content on the size and stability of the complexes using the ethidium bromide fluorescence assay, gel electrophoresis, dynamic light scattering. and fluorescence microscopy. The size and colloidal stability of deacetylated chitosan (CH/DNA) and PC-CH/DNA complexes were strongly dependent on phosphorylcholine content, charge ratios, and pH. The interaction strengths were evaluated from ethidium bromide fluorescence, and at N/P ratios higher than 5.0, no DNA release was observed in any synthesized PC-CH/DNA polyplexes by gel electrophoresis. The PC-CH/DNA polyplexes exhibited a higher resistance to aggregation compared to deacetylated chitosan (CH) at neutral pH. At low pH values highly charged chitosan and its phosphorylcholine derivatives had strong binding affinity with DNA, whereas at higher pH Values CH formed large aggregates and only C-CH derivatives were able to form small nanoparticles with hydrodynamic radii varying from 100 to 150 nm. Nanoparticles synthesized at low ionic strength with PC-CH derivatives containing moderate degrees of substitution (DS = 20% and 40%) remained stable for weeks. Photomicroscopies also confirmed that rhodamine-labeled PC(40)CH derivative nanoparticles presented higher colloidal stability than those synthesized using deacetylated chitosan. Accordingly, due to their improved physicochemical properties these phosphorylcholine-modified chitosans provide new perspectives for controlling the properties of polyplexes. (C) 2009 Elsevier B.V. All rights reserved.en
dc.description.affiliationSão Paulo State Univ, UNESP, IBILCE, Inst Biosci Humanities & Exact Sci,Dept Chem & En, BR-15054000 São Paulo, Brazil
dc.description.affiliationSão Paulo State Univ, UNESP, IBILCE, Inst Biosci Humanities & Exact Sci,Dept Phys, BR-15054000 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Sch Philosophy Sci & Humanities, Dept Chem, FCL, São Paulo, Brazil
dc.description.affiliationHop Sacre Coeur, Orthoped Res Lab, Montreal, PQ H4J 1C5, Canada
dc.description.affiliationSão Paulo State Univ, UNESP, IBILCE, Inst Biosci Humanities & Exact Sci,Dept Biol, BR-15054000 São Paulo, Brazil
dc.description.affiliationUniv Montreal, Fac Pharm, Montreal, PQ H3C 3J7, Canada
dc.description.affiliationUniv Montreal, Dept Chem, Montreal, PQ H3C 3J7, Canada
dc.description.affiliationUnespSão Paulo State Univ, UNESP, IBILCE, Inst Biosci Humanities & Exact Sci,Dept Chem & En, BR-15054000 São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, IBILCE, Inst Biosci Humanities & Exact Sci,Dept Phys, BR-15054000 São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, IBILCE, Inst Biosci Humanities & Exact Sci,Dept Biol, BR-15054000 São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 07/00339-7
dc.format.extent125-133
dc.identifierhttp://dx.doi.org/10.1016/j.jcis.2009.02.069
dc.identifier.citationJournal of Colloid and Interface Science. San Diego: Academic Press Inc. Elsevier B.V., v. 336, n. 1, p. 125-133, 2009.
dc.identifier.doi10.1016/j.jcis.2009.02.069
dc.identifier.issn0021-9797
dc.identifier.lattes1445259468526188
dc.identifier.lattes8796747160088337
dc.identifier.orcid0000-0002-0970-4288
dc.identifier.urihttp://hdl.handle.net/11449/21589
dc.identifier.wosWOS:000266845400017
dc.language.isoeng
dc.publisherAcademic Press Inc. Elsevier B.V.
dc.relation.ispartofJournal of Colloid and Interface Science
dc.relation.ispartofjcr5.091
dc.relation.ispartofsjr1,221
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectChitosanen
dc.subjectnanoparticlesen
dc.subjectGene therapyen
dc.subjectDNAen
dc.subjectPhosphorylcholineen
dc.subjectDerivatesen
dc.titlePhysicochemical characterization of nanoparticles formed between DNA and phosphorylcholine substituted chitosansen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderAcademic Press Inc. Elsevier B.V.
unesp.author.lattes1445259468526188
unesp.author.lattes8796747160088337
unesp.author.orcid0000-0002-0970-4288[5]
unesp.author.orcid0000-0002-5472-1482[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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