Expression of matrix metalloproteinases, type IV collagen, and interleukin-10 in rabbits treated with morphine after lamellar keratectomy

Página do item simplificado
dc.contributor.authorRibeiro, Alexandre P.
dc.contributor.authorSilva, Miguel L. [UNESP]
dc.contributor.authorAraujo, Rodrigo L.
dc.contributor.authorFerrucci, Danilo L.
dc.contributor.authorMineo, Tiago
dc.contributor.authorThiesen, Roberto
dc.contributor.authorBandarra, Marcio de Barros [UNESP]
dc.contributor.authorLaus, Jose L. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Fed Mato Grosso
dc.contributor.institutionColl Vet Med
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.contributor.institutionUniversidade Federal do Pampa (UNIPAMPA)
dc.date.accessioned2014-05-20T13:14:13Z
dc.date.available2014-05-20T13:14:13Z
dc.date.issued2012-05-01
dc.description.abstractPurpose To study the effects of topical administration of 1% morphine on corneal analgesia in rabbits submitted to lamellar keratectomy and to assess the expression of matrix metalloproteinase-1, metalloproteinase-2, metalloproteinase-9 (MMPs), type IV collagen, and interleukin-10 (IL-10) during the treatment. Methods Morphine group (MG) received 50 mu L of topical 1% morphine four times daily, while the control group received saline instead. Corneal touch threshold (CTT) and the wound area were assessed until corneal healing. Corneal samples were processed for routine histology, immunohistochemistry, zymography, and ELISA. Results Following keratectomy, CTT increased significantly from 6 to 96 h time points. Mean corneal re-epithelization rate and scores of leukocyte infiltration did not differ significantly between treatment groups. Immunolabeling pattern for MMP-1, MMP-9, and type IV collagen was similar in both treatment groups. In the MG, zymography indicated significantly higher levels of active MMP-2 on days 6 and 12; and in the latent MMP-9, on days 3 and 6, and in the active MMP-9, on day 6. Latent MMP-2 and MMP-9, and active MMP-9 decreased to values close to those of healthy corneas on day 12, but levels of active MMP-2 remained significantly elevated in the MG. IL-10 levels measured on days 16 were reduced as compared to those of healthy corneal tissue and returned to levels close to those of healthy corneas on day 12. Conclusion Topical morphine promoted corneal analgesia for up to 4 days and did not delay corneal re-epithelization. The re-establishment of MMPs and IL-10 to levels close to baseline values at the end of the study and the expression of type IV collagen in both groups reinforce that, with caution, 1% morphine can be used after lamellar keratectomy in rabbits.en
dc.description.affiliationSão Paulo State Univ, Dept Vet Clin & Surg, Coll Agr & Vet Sci, Jaboticabal, SP, Brazil
dc.description.affiliationUniv Fed Mato Grosso, Dept Vet Clin, Coll Agron Vet Med & Anim Sci, Cuiaba, MT, Brazil
dc.description.affiliationColl Vet Med, Dept Vet Anesthesia & Surg, Aracatuba, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Dept Immunol, Campinas, Brazil
dc.description.affiliationUniversidade Federal de Uberlândia (UFU), Dept Immunol, BR-38400 Uberlandia, MG, Brazil
dc.description.affiliationFed Univ Pampa, Uruguaiana, RS, Brazil
dc.description.affiliationSão Paulo State Univ, Dept Vet Pathol, Coll Agr & Vet Sci, Jaboticabal, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Dept Vet Clin & Surg, Coll Agr & Vet Sci, Jaboticabal, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Dept Vet Pathol, Coll Agr & Vet Sci, Jaboticabal, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 07/58783-0
dc.description.sponsorshipIdFAPESP: 07/06519-7
dc.format.extent153-163
dc.identifierhttp://dx.doi.org/10.1111/j.1463-5224.2011.00954.x
dc.identifier.citationVeterinary Ophthalmology. Malden: Wiley-blackwell, v. 15, n. 3, p. 153-163, 2012.
dc.identifier.doi10.1111/j.1463-5224.2011.00954.x
dc.identifier.issn1463-5216
dc.identifier.urihttp://hdl.handle.net/11449/1747
dc.identifier.wosWOS:000303158000003
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofVeterinary Ophthalmology
dc.relation.ispartofjcr1.083
dc.relation.ispartofsjr0,692
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCorneal wound healingen
dc.subjectInflammationen
dc.subjectopioidsen
dc.subjectPainen
dc.subjectRabbitsen
dc.subjectside effectsen
dc.titleExpression of matrix metalloproteinases, type IV collagen, and interleukin-10 in rabbits treated with morphine after lamellar keratectomyen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-blackwell
unesp.author.orcid0000-0002-2339-2743[5]
unesp.author.orcid0000-0002-7568-2275[4]
unesp.author.orcid0000-0002-8922-2886[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

Arquivos

Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Clínica e Cirurgia Veterinária - FCAV
Artigos - Patologia Veterinária - FCAV