Unraveling a lignocellulose-decomposing bacterial consortium from soil associated with dry sugarcane straw by genomic-centered metagenomics

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dc.contributor.authorWeiss, Bruno [UNESP]
dc.contributor.authorSouza, Anna Carolina Oliveira [UNESP]
dc.contributor.authorConstancio, Milena Tavares Lima [UNESP]
dc.contributor.authorAlvarenga, Danillo Oliveira [UNESP]
dc.contributor.authorPylro, Victor S.
dc.contributor.authorAlves, Lucia M. Carareto [UNESP]
dc.contributor.authorVarani, Alessandro M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Lavras (UFLA)
dc.date.accessioned2021-06-25T10:29:41Z
dc.date.available2021-06-25T10:29:41Z
dc.date.issued2021-05-01
dc.description.abstractSecond-generation biofuel production is in high demand, but lignocellulosic biomass’ complexity impairs its use due to the vast diversity of enzymes necessary to execute the complete saccharification. In nature, lignocellulose can be rapidly deconstructed due to the division of biochemical labor effectuated in bacterial communities. Here, we analyzed the lignocellulolytic potential of a bacterial consortium obtained from soil and dry straw leftover from a sugarcane milling plant. This consortium was cultivated for 20 weeks in aerobic conditions using sugarcane bagasse as a sole carbon source. Scanning electron microscopy and chemical analyses registered modification of the sugarcane fiber’s appearance and biochemical composition, indicating that this consortium can deconstruct cellulose and hemicellulose but no lignin. A total of 52 metagenome-assembled genomes from eight bacterial classes (Actinobacteria, Alphaproteobacteria, Bacilli, Bacteroidia, Cytophagia, Gammaproteobacteria, Oligoflexia, and Thermoleophilia) were recovered from the consortium, in which ~46% of species showed no relevant modification in their abundance during the 20 weeks of cultivation, suggesting a mostly stable consortium. Their CAZymes repertoire indicated that many of the most abundant species are known to deconstruct lignin (e.g., Chryseobacterium) and carry sequences related to hemicellulose and cellulose deconstruction (e.g., Chitinophaga, Niastella, Niabella, and Siphonobacter). Taken together, our results unraveled the bacterial diversity, enzymatic potential, and effectiveness of this lignocellulose-decomposing bacterial consortium.en
dc.description.affiliationDepartament of Technology School of Agricultural and Veterinary Sciences São Paulo State University (UNESP)
dc.description.affiliationGraduate Program in Agricultural and Livestock Microbiology School of Agricultural and Veterinary Sciences São Paulo State University (UNESP)
dc.description.affiliationMicrobial Ecology and Bioinformatics Laboratory Department of Biology Federal University of Lavras (UFLA)
dc.description.affiliationUnespDepartament of Technology School of Agricultural and Veterinary Sciences São Paulo State University (UNESP)
dc.description.affiliationUnespGraduate Program in Agricultural and Livestock Microbiology School of Agricultural and Veterinary Sciences São Paulo State University (UNESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCAPES: 001
dc.description.sponsorshipIdFAPESP: 10/17520-9
dc.description.sponsorshipIdFAPESP: 16/16624-1
dc.description.sponsorshipIdCNPq: 302085/2017-3
dc.description.sponsorshipIdCNPq: 303061/2019-7
dc.identifierhttp://dx.doi.org/10.3390/microorganisms9050995
dc.identifier.citationMicroorganisms, v. 9, n. 5, 2021.
dc.identifier.doi10.3390/microorganisms9050995
dc.identifier.issn2076-2607
dc.identifier.scopus2-s2.0-85105235036
dc.identifier.urihttp://hdl.handle.net/11449/206295
dc.language.isoeng
dc.relation.ispartofMicroorganisms
dc.sourceScopus
dc.subjectBiofuels
dc.subjectBiotechnology
dc.subjectCommunity dynamics
dc.subjectLignocellulose
dc.subjectMetabolic modeling
dc.titleUnraveling a lignocellulose-decomposing bacterial consortium from soil associated with dry sugarcane straw by genomic-centered metagenomicsen
dc.typeArtigo

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