Role of Paracoccidioides brasiliensis cell wall fraction containing β-glucan in tumor necrosis factor-alpha production by human monocytes: Correlation with fungicidal activity

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dc.contributor.authorAnjos, A. R. [UNESP]
dc.contributor.authorCalvi, S. A. [UNESP]
dc.contributor.authorFerracini, R.
dc.contributor.authorPeraçoli, M. T.S. [UNESP]
dc.contributor.authorSilva, C. L.
dc.contributor.authorSoares, A. M.V.C. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2022-04-28T19:55:35Z
dc.date.available2022-04-28T19:55:35Z
dc.date.issued2002-01-01
dc.description.abstractThe polysaccharide fraction of Paracoccidioides brasiliensis mycelial cell wall (F1 fraction), the active component of which is composed of β-glucan, was investigated in regard to the activation of human monocytes for fungal killing. The cells were primed with interferon-γ (IFN-γ) or F1 (100 and 200 μg ml-1) or F1 (100 and 200 μg ml-1) plus IFN-γ for 24 h and then evaluated for H2O2release. In other experiments, the cells were pretreated with the same stimuli, challenged with a virulent strain of P. brasiliensis and evaluated for fungicidal activity and levels of tumor necrosis factor (TNF-α) in the supernatants. F1 increased the levels of H2O2in a similar manner to IFN-γ. However, a synergistic effect between these two activators was not detected. On the contrary, a significant fungicidal activity was only obtained after priming with IFN-γ plus F1. This higher activity was associated with high levels of TNF-α in the supernatants of the cocultures. Overall, P. brasiliensis F1 fraction induced human monocytes to release relatively high levels of TNF-α, which, in combination with IFN-γ, is responsible for the activation of human monocytes for effective killing of P. brasiliensis.en
dc.description.affiliationDepartment of Microbiology and Immunology Biosciences Institute São Paulo State University-Botucatu, SP
dc.description.affiliationDepartment of Parasitology Microbiology and Immunology School of Medicine of Ribeirão Preto University of São Paulo, SP
dc.description.affiliationUnespDepartment of Microbiology and Immunology Biosciences Institute São Paulo State University-Botucatu, SP
dc.format.extent377-382
dc.identifierhttp://dx.doi.org/10.1080/mmy.40.4.377.382
dc.identifier.citationMedical Mycology, v. 40, n. 4, p. 377-382, 2002.
dc.identifier.doi10.1080/mmy.40.4.377.382
dc.identifier.issn1369-3786
dc.identifier.scopus2-s2.0-0036668543
dc.identifier.urihttp://hdl.handle.net/11449/224277
dc.language.isoeng
dc.relation.ispartofMedical Mycology
dc.sourceScopus
dc.subjectFungicidal activity
dc.subjectMonocyte
dc.subjectParacoccidioides brasiliensis
dc.subjectTNF
dc.subjectβ-Glucan
dc.titleRole of Paracoccidioides brasiliensis cell wall fraction containing β-glucan in tumor necrosis factor-alpha production by human monocytes: Correlation with fungicidal activityen
dc.typeArtigo

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