Catecholaminergic neurons in the comissural region of the nucleus of the solitary tract modulate hyperosmolality-induced responses

Página do item simplificado
dc.contributor.authorFreiria-Oliveira, André H. [UNESP]
dc.contributor.authorBlanch, Graziela T. [UNESP]
dc.contributor.authorPedrino, Gustavo R.
dc.contributor.authorCravo, Sergio L.
dc.contributor.authorMurphy, David
dc.contributor.authorMenani, José V. [UNESP]
dc.contributor.authorColombari, Débora S. A. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Goias
dc.contributor.institutionUniversidade Federal de São Paulo
dc.contributor.institutionUniversity of Malaya
dc.date.accessioned2015-12-07T15:40:25Z
dc.date.available2015-12-07T15:40:25Z
dc.date.issued2015-11-01
dc.description.abstractNoradrenergic A2 neurons of the nucleus of the solitary tract (NTS) have been suggested to contribute to body fluid homeostasis and cardiovascular regulation. In the present study, we investigated the effects of lesions of A2 neurons of the commissural NTS (cNTS) on the c-Fos expression in neurons of the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei, arterial pressure, water intake, and urinary excretion in rats with plasma hyperosmolality produced by intragastric 2 M NaCl (2 ml/rat). Male Holtzman rats (280-320 g) received an injection of anti-dopamine-β-hydroxylase-saporin (12.6 ng/60 nl; cNTS/A2-lesion, n = 28) or immunoglobulin G (IgG)-saporin (12.6 ng/60 nl; sham, n = 24) into the cNTS. The cNTS/A2 lesions increased the number of neurons expressing c-Fos in the magnocellular PVN in rats treated with hypertonic NaCl (90 ± 13, vs. sham: 47 ± 20; n = 4), without changing the number of neurons expressing c-Fos in the parvocellular PVN or in the SON. Contrary to sham rats, intragastric 2 M NaCl also increased arterial pressure in cNTS/A2-lesioned rats (16 ± 3, vs. sham: 2 ± 2 mmHg 60 min after the intragastric load; n = 9), an effect blocked by the pretreatment with the vasopressin antagonist Manning compound (0 ± 3 mmHg; n = 10). In addition, cNTS/A2 lesions enhanced hyperosmolality-induced water intake (10.5 ± 1.4, vs. sham: 7.7 ± 0.8 ml/60 min; n = 8-10), without changing renal responses to hyperosmolality. The results suggest that inhibitory mechanisms dependent on cNTS/A2 neurons reduce water intake and vasopressin-dependent pressor response to an acute increase in plasma osmolality.en
dc.description.affiliationDepartment of Pathology and Physiology, School of Dentistry, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil
dc.description.affiliationDepartment of Physiological Sciences, Federal University of Goias, Goiania, Goias, Brazil
dc.description.affiliationDepartment of Physiology, Escola Paulista de Medicina, Universidade Federal de São Paulo (USP), São Paulo, Brazil
dc.description.affiliationHenry Welcome Laboratories for Integrative Neuroscience and Endocrinology, University of Bristol, Bristol, United Kingdom
dc.description.affiliationDepartment of Physiology, University of Malaya, Kuala Lumpur, Malaysia
dc.description.affiliationUnespDepartment of Pathology and Physiology, School of Dentistry, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipBBSRC
dc.description.sponsorshipIdBBSRC: BB/J015415/1
dc.format.extent1082-1091
dc.identifierhttp://dx.doi.org/10.1152/ajpregu.00432.2014
dc.identifier.citationAmerican Journal Of Physiology. Regulatory, Integrative And Comparative Physiology, v. 309, n. 9, p. 1082-1091, 2015.
dc.identifier.doi10.1152/ajpregu.00432.2014
dc.identifier.issn1522-1490
dc.identifier.pubmed26333788
dc.identifier.urihttp://hdl.handle.net/11449/131679
dc.language.isoeng
dc.publisherThe American Physiological Society.
dc.relation.ispartofAmerican Journal Of Physiology. Regulatory, Integrative And Comparative Physiology
dc.relation.ispartofsjr1,550
dc.rights.accessRightsAcesso restrito
dc.sourcePubMed
dc.subjectBlood pressureen
dc.subjectC-fos expressionen
dc.subjectOsmoreceptoren
dc.subjectThirsten
dc.subjectVasopressinen
dc.titleCatecholaminergic neurons in the comissural region of the nucleus of the solitary tract modulate hyperosmolality-induced responsesen
dc.typeArtigo
dcterms.rightsHolderThe American Physiological Society
unesp.author.orcid0000-0003-1167-4441[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

Arquivos

Coleções

Artigos - Fisiologia e Patologia - FOAR