Food restriction promotes downregulation of myocardial L-type Ca2+ channels

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dc.contributor.authorDe Tomasi, Loreta Casquel [UNESP]
dc.contributor.authorBruno, Alessandro [UNESP]
dc.contributor.authorSugizaki, Mario Mateus [UNESP]
dc.contributor.authorLima-Leopoldo, Ana Paula [UNESP]
dc.contributor.authorNascimento, Andre Ferreira [UNESP]
dc.contributor.authorde Oliveira Junior, Silvio Assis [UNESP]
dc.contributor.authorPinotti, Matheus Fecchio [UNESP]
dc.contributor.authorPadovani, Carlos Roberto [UNESP]
dc.contributor.authorLeopoldo, Andre Soares [UNESP]
dc.contributor.authorCicogna, Antonio Carlos [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:33:16Z
dc.date.available2014-05-20T13:33:16Z
dc.date.issued2009-06-01
dc.description.abstractFood restriction (FR) has been shown to impair myocardial performance. However, the mechanisms behind these changes in myocardial function due to FR remain unknown. Since myocardial L-type Ca2+ channels may contribute to the cardiac dysfunction, we examined the influence of FR on L-type Ca2+ channels. Male 60-day-old Wistar rats were fed a control or a restricted diet (daily intake reduced to 50% of the amount of food consumed by the control group) for 90 days. Myocardial performance was evaluated in isolated left ventricular papillary muscles. The function of myocardial L-type Ca2+ channels was determined by using a pharmacological Ca2+ channel blocker, and changes in the number of channels were evaluated by mRNA and protein expression. FR decreased final body weights, as well as weights of the left and right ventricles. The Ca2+ channel blocker diltiazem promoted a higher blockade on developed tension in FR groups than in controls. The protein content of L-type Ca2+ channels was significantly diminished in FR rats, whereas the mRNA expression was similar between groups. These results suggest that the myocardial dysfunction observed in previous studies with FR animals could be caused by downregulation of L-type Ca2+ channels.en
dc.description.affiliationUNESP State Univ Julio Mesquita Filho, Sch Med, Dept Clin Cardiol, BR-18618000 São Paulo, Brazil
dc.description.affiliationUNESP State Univ Julio Mesquita Filho, Inst Biol Sci, Dept Biostat, BR-18618000 São Paulo, Brazil
dc.description.affiliationUnespUNESP State Univ Julio Mesquita Filho, Sch Med, Dept Clin Cardiol, BR-18618000 São Paulo, Brazil
dc.description.affiliationUnespUNESP State Univ Julio Mesquita Filho, Inst Biol Sci, Dept Biostat, BR-18618000 São Paulo, Brazil
dc.format.extent426-431
dc.identifierhttp://dx.doi.org/10.1139/Y09-025
dc.identifier.citationCanadian Journal of Physiology and Pharmacology. Ottawa: Canadian Science Publishing, Nrc Research Press, v. 87, n. 6, p. 426-431, 2009.
dc.identifier.doi10.1139/Y09-025
dc.identifier.issn0008-4212
dc.identifier.lattes9418970103564137
dc.identifier.lattes8727897080522289
dc.identifier.urihttp://hdl.handle.net/11449/11372
dc.identifier.wosWOS:000267075200003
dc.language.isoeng
dc.publisherCanadian Science Publishing, Nrc Research Press
dc.relation.ispartofCanadian Journal of Physiology and Pharmacology
dc.relation.ispartofjcr2.210
dc.relation.ispartofsjr0,724
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectfood restrictionen
dc.subjectpapillary muscleen
dc.subjectL-type calcium channels functionen
dc.subjectL-type calcium mRNAen
dc.subjectL-type calcium proteinen
dc.subjectdiltiazemen
dc.subjectrateen
dc.titleFood restriction promotes downregulation of myocardial L-type Ca2+ channelsen
dc.typeArtigo
dcterms.licensehttp://www.nrcresearchpress.com/page/authors/information/rights
dcterms.rightsHolderCanadian Science Publishing, Nrc Research Press
unesp.author.lattes9418970103564137[10]
unesp.author.lattes8727897080522289[8]
unesp.author.orcid0000-0002-7719-9682[8]
unesp.author.orcid0000-0002-4402-6523[10]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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