Crystallization and preliminary X-ray diffraction analysis of XAC1151, a small heat-shock protein from Xanthomonas axonopodis pv. citri belonging to the alpha-crystallin family

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dc.contributor.authorHilario, E.
dc.contributor.authorTeixeira, E. C.
dc.contributor.authorPedroso, G. A.
dc.contributor.authorBertolini, Maria Celia [UNESP]
dc.contributor.authorMedrano, F. J.
dc.contributor.institutionCtr Biol Mol Estrrutural
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:21:46Z
dc.date.available2014-05-20T15:21:46Z
dc.date.issued2006-05-01
dc.description.abstractThe hspA gene (XAC1151) from Xanthomonas axonopodis pv. citri encodes a protein of 158 amino acids that belongs to the small heat-shock protein ( sHSP) family of proteins. These proteins function as molecular chaperones by preventing protein aggregation. The protein was crystallized using the sitting-drop vapour-diffusion method in the presence of ammonium phosphate. X-ray diffraction data were collected to 1.65 angstrom resolution using a synchrotron-radiation source. The crystal belongs to the rhombohedral space group R3, with unit-cell parameters a = b = 128.7, c = 55.3 angstrom. The crystal structure was solved by molecular-replacement methods. Structure refinement is in progress.en
dc.description.affiliationCtr Biol Mol Estrrutural, Dept Cristalog Prot, Lab Nacl Luz Sincrotron, BR-13084971 Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Quim, Dept Bioquim & Tecnol Quim, Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Quim, Dept Bioquim & Tecnol Quim, Araraquara, SP, Brazil
dc.format.extent446-448
dc.identifierhttp://dx.doi.org/10.1107/S174430910601219X
dc.identifier.citationActa Crystallographica Section F-structural Biology and Crystallization Communications. Oxford: Blackwell Publishing, v. 62, p. 446-448, 2006.
dc.identifier.doi10.1107/S174430910601219X
dc.identifier.issn1744-3091
dc.identifier.lattes8817669953838863
dc.identifier.urihttp://hdl.handle.net/11449/32886
dc.identifier.wosWOS:000237159000007
dc.language.isoeng
dc.publisherBlackwell Publishing
dc.relation.ispartofActa Crystallographica Section F: Structural Biology and Crystallization Communications
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleCrystallization and preliminary X-ray diffraction analysis of XAC1151, a small heat-shock protein from Xanthomonas axonopodis pv. citri belonging to the alpha-crystallin familyen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderBlackwell Publishing
unesp.author.lattes8817669953838863
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt

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