Kinetic characterization of a membrane-specific ATPase from rat osseous plate and its possible significance on endochondral ossification

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dc.contributor.authorPizauro, J. M.
dc.contributor.authorDemenis, M. A.
dc.contributor.authorCiancaglini, P.
dc.contributor.authorLeone, F. A.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:17:32Z
dc.date.available2014-05-20T13:17:32Z
dc.date.issued1998-01-05
dc.description.abstractTreatment with phosphatidylinositol-specific phospholipase C of rat osseous plate membranes released up to 90-95% of alkaline phosphatase, but a specific ATPase activity (optimum pH = 7.5) remained bound to the membrane. The hydrolysis of ATP by this ATPase was negligible in the absence of magnesium or calcium ions. However, at millimolar concentrations of magnesium and calcium ions, the membrane-specific ATPase activity increased to about 560-600 U/mg, exhibiting two classes of ATP-hydrolysing sites, and site-site interactions. GTP, UTP, ITP, and CTP were also hydrolyzed by the membrane-specific ATPase. Oligomycin, ouabain, bafilomycin A(1), thapsigargin, omeprazole, ethacrynic acid and EDTA slightly affected membrane-specific ATPase activity while vanadate produced a 18% inhibition. The membrane-specific ATPase activity was insensitive to theophylline, but was inhibited 40% by levamisole. These data suggested that the membrane-specific ATPase activity present in osseous plate membranes, and alkaline phosphatase, were different proteins. (C) 1998 Elsevier B.V. B.V.en
dc.description.affiliationUSP, Fac Filosofia Ciências & Letras Ribeirao Pret, Dept Quim, BR-14040901 Ribeirao Preto, SP, Brazil
dc.description.affiliationUNESP, Fac Ciências Agr & Vet, Dept Tecnol, Jaboticabal, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Ciências Agr & Vet, Dept Tecnol, Jaboticabal, SP, Brazil
dc.format.extent108-114
dc.identifierhttp://dx.doi.org/10.1016/S0005-2736(97)00174-0
dc.identifier.citationBiochimica Et Biophysica Acta-biomembranes. Amsterdam: Elsevier B.V., v. 1368, n. 1, p. 108-114, 1998.
dc.identifier.doi10.1016/S0005-2736(97)00174-0
dc.identifier.fileWOS000071507700012.pdf
dc.identifier.issn0005-2736
dc.identifier.urihttp://hdl.handle.net/11449/3973
dc.identifier.wosWOS:000071507700012
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBiochimica et Biophysica Acta: Biomembranes
dc.relation.ispartofjcr3.438
dc.relation.ispartofsjr1,495
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectATPasept
dc.subjectOsseous platept
dc.subjectphosphatidylinositol-specific phospholipase Cpt
dc.subjectendochondral ossificationpt
dc.subjectAlkaline phosphatasept
dc.titleKinetic characterization of a membrane-specific ATPase from rat osseous plate and its possible significance on endochondral ossificationen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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