Comparative RH Maps of the River Buffalo and Bovine Y Chromosomes

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dc.contributor.authorStafuzza, N. B. [UNESP]
dc.contributor.authorAbbassi, H.
dc.contributor.authorGrant, J. R.
dc.contributor.authorRodrigues-Filho, E. A. [UNESP]
dc.contributor.authorIanella, P. [UNESP]
dc.contributor.authorKadri, S. M. [UNESP]
dc.contributor.authorAmarante, M. V. [UNESP]
dc.contributor.authorStohard, P.
dc.contributor.authorWomack, J. E.
dc.contributor.authorde Leon, F. A. Ponce
dc.contributor.authorAmaral, M. E. J. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Minnesota
dc.contributor.institutionUniv Alberta
dc.contributor.institutionTexas A&M Univ
dc.date.accessioned2014-05-20T13:52:59Z
dc.date.available2014-05-20T13:52:59Z
dc.date.issued2009-01-01
dc.description.abstractRadiation hybrid maps were constructed for river buffalo and cattle Y chromosomes. A total of 41 cattle-derived Y-chromosome molecular markers were selected and tested with 2 previously described 5,000-rad whole-genome radiation hybrid (RH) panels (river buffalo - BBURH(5000) and cattle - BTARH(5000)) for generation of maps. Among the initial 41 selected markers, a subset of 26 markers generated PCR products suitable for scoring with the BBURH(5000) panel. of these, 19 markers (73%) were distributed in 1 linkage group spanning 341.3 cR. Retention frequencies (RF) for individual markers ranged from 17.8% for SMCY to 56.7% for BTY1, with an average RF of 37.6%. From the selected markers, 37 generated reliable scores using the BTARH(5000) panel. The newly constructed BTAY RH map contains 28 markers distributed within 1 linkage group. Twenty- four of these markers had been previously mapped on BTAY using a 7,000- rad cattlehamster WG- RH panel and 4 markers were mapped for the first time (ZFY, SeqRep, RepSeqS4 and BTY1). The length of the BTAY RH map was estimated to be 602.4 cR. Retention fre- quencies for individual mapped markers ranged from 10% (INRA126) to 63.3% (SeqRep), with an average RF of 35.3%. RH marker positions along the Y chromosome were compared between BBUY and BTAY, which revealed differences in the order of some of the markers. The BBUY pseudoautosomal region (PAR) is delineated by 3 BTAY PAR markers (MAF45, TGLA325 and UMN2008). These markers are telomeric in both species but are not found in the same order. Here we have demonstrated the effective use of bovine Y chromosome markers for the development of the first BBUY RH map. Likewise, these set of markers can be used for comparative assessment of Y chromosomes in other members of the Bovidae family. Copyright (C) 2009 S. Karger AG, Baselen
dc.description.affiliationSão Paulo State Univ, UNESP, IBILCE, Dept Biol, Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUniv Minnesota, Dept Anim Sci, Coll Food Agr & Nat Resource Sci, St Paul, MN 55108 USA
dc.description.affiliationUniv Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB, Canada
dc.description.affiliationSão Paulo State Univ, UNESP, Inst Biociencias, Dept Parasitol, Botucatu, SP, Brazil
dc.description.affiliationTexas A&M Univ, Dept Vet Pathobiol, College Stn, TX USA
dc.description.affiliationUnespSão Paulo State Univ, UNESP, IBILCE, Dept Biol, Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Inst Biociencias, Dept Parasitol, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipNSF-USA
dc.description.sponsorshipHatch project
dc.description.sponsorshipIdFAPESP: 02/10150-5
dc.description.sponsorshipIdFAPESP: 04/08768-6
dc.description.sponsorshipIdNSF-USA: OISE-0405743
dc.description.sponsorshipIdHatch project: MIN-16-019
dc.format.extent132-138
dc.identifierhttp://dx.doi.org/10.1159/000245912
dc.identifier.citationCytogenetic and Genome Research. Basel: Karger, v. 126, n. 1-2, p. 132-138, 2009.
dc.identifier.doi10.1159/000245912
dc.identifier.issn1424-8581
dc.identifier.lattes0175083848055466
dc.identifier.orcid0000-0002-5929-1223
dc.identifier.urihttp://hdl.handle.net/11449/18903
dc.identifier.wosWOS:000272605100012
dc.language.isoeng
dc.publisherKarger
dc.relation.ispartofCytogenetic and Genome Research
dc.relation.ispartofjcr1.587
dc.relation.ispartofsjr0,748
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBovineen
dc.subjectComparative RH mapen
dc.subjectRiver buffaloen
dc.subjectY chromosomeen
dc.titleComparative RH Maps of the River Buffalo and Bovine Y Chromosomesen
dc.typeArtigo
dcterms.licensehttp://www.karger.com/Services/RightsPermissions
dcterms.rightsHolderKarger
unesp.author.lattes0175083848055466
unesp.author.orcid0000-0001-6432-2330[1]
unesp.author.orcid0000-0002-5929-1223[7]
unesp.author.orcid0000-0002-6651-2987[5]
unesp.author.orcid0000-0001-8645-553X[10]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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