Contribution to characterization of skin field cancerization activity: morphometric, chromatin texture, proliferation, and apoptosis aspects

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dc.contributor.authorMiola, Anna Carolina [UNESP]
dc.contributor.authorCastilho, Mariana Anteghini [UNESP]
dc.contributor.authorSchmitt, Juliano Vilaverde [UNESP]
dc.contributor.authorAlencar Marques, Mariangela Esther [UNESP]
dc.contributor.authorMiot, Helio Amante [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInst Lauro Souza Lima
dc.date.accessioned2020-12-10T19:48:05Z
dc.date.available2020-12-10T19:48:05Z
dc.date.issued2019-11-01
dc.description.abstractBackground: A skin field cancerization is a cutaneous area with subclinical changes resultant from chronic sun exposure, with a higher predisposition to development of pre-neoplastic and neoplastic lesions. So far, there are no well-defined objective parameters that can indicate their degree of activity. Objectives: To describe and compare morphometric aspects and expression of factors related to apoptosis and cell proliferation in actinic keratosis (AK), in both photoexposed and photoprotected epidermis. Methods: A cross-sectional study of patients with actinic keratosis in the forearms, biopsied at two points: the actinic keratosis and the axillary region. The biopsies of the actinic keratosis, perilesional area, and axilla were evaluated through keratinocyte intraepithelial neoplasia (KIN), and immunohistochemistry of p53, survivin, and Ki67. Nuclear morphometry of basal layer cells was performed through digital image analysis: entropy, area, perimeter, Ra, fractal dimension, circularity, color intensity, and largest diameter. Results: There were 13 patients included and 38 actinic keratosis biopsied. In morphometry, 1039 nuclei were analyzed, of which 228 represented axillary skin, 396 demonstrated actinic keratosis, and 415 represented the peritesional area to the actinic keratosis. There was a significant difference (p <0.05) in all variables tested for the topographies evaluated. A significant correlation was identified between nucellar morphometric elements, KIN, proliferation markers, and apoptosis. Joint patterns of p53, Ki67, and KIN discriminated the topographies sampled. Study limitations: This was a cross-sectional study with a small number of patients. Conclusions: There are patterns of proliferation, resistance to apoptosis, and different cellular morphometrics between photoprotected skin and photoexposed skin. The joint expression of p53, Ki67, and KIN can characterize skin field cancerization activity. (C) 2019 Sociedade Brasileira de Dermatologia. Published by Elsevier Espana, S.L.U.en
dc.description.affiliationUniv Estadual Paulista, Fac Med Botucatu, Dept Dermatol & Radiotherapy, Botucatu, SP, Brazil
dc.description.affiliationInst Lauro Souza Lima, Dept Dermatol, Bauru, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Med Botucatu, Discipline Radiol & Diagnost Imaging, Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Med Botucatu, Dept Pathol, Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Med Botucatu, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Med Botucatu, Dept Dermatol & Radiotherapy, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Med Botucatu, Discipline Radiol & Diagnost Imaging, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Med Botucatu, Dept Pathol, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Med Botucatu, Botucatu, SP, Brazil
dc.format.extent698-703
dc.identifierhttp://dx.doi.org/10.1016/j.abd.2019.03.003
dc.identifier.citationAnais Brasileiros De Dermatologia. Rio De Janeiro Rj: Soc Brasileira Dermatologia, v. 94, n. 6, p. 698-703, 2019.
dc.identifier.doi10.1016/j.abd.2019.03.003
dc.identifier.fileS0365-05962019000600698.pdf
dc.identifier.issn0365-0596
dc.identifier.scieloS0365-05962019000600698
dc.identifier.urihttp://hdl.handle.net/11449/196536
dc.identifier.wosWOS:000509999500008
dc.language.isoeng
dc.publisherSoc Brasileira Dermatologia
dc.relation.ispartofAnais Brasileiros De Dermatologia
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectCarcinoma
dc.subjectsquamous cell
dc.subjectKeratosis
dc.subjectactinic
dc.subjectSkin neoplasms
dc.titleContribution to characterization of skin field cancerization activity: morphometric, chromatin texture, proliferation, and apoptosis aspectsen
dc.typeArtigo
dcterms.rightsHolderSoc Brasileira Dermatologia
unesp.author.orcid0000-0002-2596-9294[5]

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