Effect of recombinant glutathione S-transferase as vaccine antigen against Rhipicephalus appendiculatus and Rhipicephalus sanguineus infestation

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dc.contributor.authorSabadin, Gabriela Alves
dc.contributor.authorParizi, Luís Fernando
dc.contributor.authorKiio, Irene
dc.contributor.authorXavier, Marina Amaral
dc.contributor.authorda Silva Matos, Renata [UNESP]
dc.contributor.authorCamargo-Mathias, Maria Izabel [UNESP]
dc.contributor.authorGithaka, Naftaly Wang'ombe
dc.contributor.authorNene, Vish
dc.contributor.authorda Silva Vaz, Itabajara
dc.contributor.institutionUniversidade Federal do Rio Grande do Sul
dc.contributor.institutionInternational Livestock Research Institute (ILRI)
dc.contributor.institutionUniversity of Nairobi
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInstituto Nacional de Ciência e Tecnologia em Entomologia Molecular
dc.date.accessioned2018-12-11T16:50:08Z
dc.date.available2018-12-11T16:50:08Z
dc.date.issued2017-12-04
dc.description.abstractThe ticks Rhipicephalus appendiculatus and Rhipicephalus sanguineus are the main vectors of Theileria parva and Babesia spp. in cattle and dogs, respectively. Due to their impact in veterinary care and industry, improved methods against R. appendiculatus and R. sanguineus parasitism are under development, including vaccines. We have previously demonstrated the induction of a cross-protective humoral response against Rhipicephalus microplus following vaccination with recombinant glutathione S-transferase from Haemaphysalis longicornis tick (rGST-Hl), suggesting that this protein could control tick infestations. In the present work, we investigated the effect of rGST-Hl vaccine against R. appendiculatus and R. sanguineus infestation in rabbits. In silico analysis revealed that GST from H. longicornis, R. appendiculatus and R. sanguineus have >80% protein sequence similarity, and multiple conserved antigenic sites. After the second vaccine dose, rGST-Hl-immunized rabbits showed elevated antibody levels which persisted until the end of experiment (75 and 60 days for R. appendiculatus and R. sanguineus, respectively). Western blot assays demonstrated cross-reactivity between anti-rGST-Hl antibodies and native R. appendiculatus and R. sanguineus GST extracts from ticks at different life stages. Vaccination with rGST-Hl decreased the number, weight, and fertility of engorged R. appendiculatus adults, leading to an overall vaccine efficacy of 67%. Interestingly, histological analysis of organ morphology showed damage to salivary glands and ovaries of R. appendiculatus adult females fed on vaccinated animals. In contrast, rGST-Hl vaccination did not affect R. appendiculatus nymphs, and it was ineffective against R. sanguineus across the stages of nymph and adult. Taken together, our results show the potential application of rGST-Hl as an antigen in anti-tick vaccine development, however indicating a broad difference in efficacy among tick species.en
dc.description.affiliationCentro de Biotecnologia Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves, 9500
dc.description.affiliationInternational Livestock Research Institute (ILRI), PO Box 30709-00100
dc.description.affiliationDepartment of Biochemistry School of Medicine University of Nairobi, P.O. Box 30197-00100
dc.description.affiliationInstituto de Biociências UNESP-Universidade Estadual Paulista
dc.description.affiliationFaculdade de Veterinária Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves, 9090
dc.description.affiliationInstituto Nacional de Ciência e Tecnologia em Entomologia Molecular
dc.description.affiliationUnespInstituto de Biociências UNESP-Universidade Estadual Paulista
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Rio Grande do Sul
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
dc.format.extent6649-6656
dc.identifierhttp://dx.doi.org/10.1016/j.vaccine.2017.10.026
dc.identifier.citationVaccine, v. 35, n. 48, p. 6649-6656, 2017.
dc.identifier.doi10.1016/j.vaccine.2017.10.026
dc.identifier.file2-s2.0-85031819799.pdf
dc.identifier.issn1873-2518
dc.identifier.issn0264-410X
dc.identifier.scopus2-s2.0-85031819799
dc.identifier.urihttp://hdl.handle.net/11449/170293
dc.language.isoeng
dc.relation.ispartofVaccine
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCross-reaction
dc.subjectGST
dc.subjectHaemaphysalis longicornis
dc.subjectRhipicephalus microplus
dc.subjectTick
dc.subjectVaccine
dc.titleEffect of recombinant glutathione S-transferase as vaccine antigen against Rhipicephalus appendiculatus and Rhipicephalus sanguineus infestationen
dc.typeArtigo

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