Inhibition of Neurotoxic Secretory Phospholipases A(2) Enzymatic, Edematogenic, and Myotoxic Activities by Harpalycin 2, an Isoflavone Isolated from Harpalyce brasiliana Benth

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dc.contributor.authorXimenes, Rafael M.
dc.contributor.authorRabello, Marcelo M.
dc.contributor.authorAraujo, Renata M.
dc.contributor.authorSilveira, Edilberto R.
dc.contributor.authorFagundes, Fabio H. R. [UNESP]
dc.contributor.authorDiz-Filho, Eduardo B. S. [UNESP]
dc.contributor.authorBuzzo, Simone C. [UNESP]
dc.contributor.authorSoares, Veronica C. G. [UNESP]
dc.contributor.authorToyama, Daniela de O.
dc.contributor.authorGaeta, Henrique H. [UNESP]
dc.contributor.authorHernandes, Marcelo Z.
dc.contributor.authorMonteiro, Helena S. A.
dc.contributor.authorToyama, Marcos H. [UNESP]
dc.contributor.institutionUniversidade Federal do Ceará (UFC)
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.contributor.institutionUniversidade Federal do Rio Grande do Norte (UFRN)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Presbiteriana Mackenzie
dc.date.accessioned2014-05-20T13:12:24Z
dc.date.available2014-05-20T13:12:24Z
dc.date.issued2012-01-01
dc.description.abstractSecretory phospholipases A(2) (sPLA(2)) exert proinflammatory actions through lipid mediators. These enzymes have been found to be elevated in many inflammatory disorders such as rheumatoid arthritis, sepsis, and atherosclerosis. The aim of this study was to evaluate the effect of harpalycin 2 (Har2), an isoflavone isolated from Harpalyce brasiliana Benth., in the enzymatic, edematogenic, and myotoxic activities of sPLA2 from Bothrops pirajai, Crotalus durissus terrificus, Apis mellifera, and Naja naja venoms. Har2 inhibits all sPLA(2) tested. PrTX-III (B. pirajai venom) was inhibited at about 58.7%, Cdt F15 (C. d. terrificus venom) at 78.8%, Apis (from bee venom) at 87.7%, and Naja (N. naja venom) at 88.1%. Edema induced by exogenous sPLA(2) administration performed in mice paws showed significant inhibition by Har2 at the initial step. In addition, Har2 also inhibited the myotoxic activity of these sPLA(2)s. In order to understand how Har2 interacts with these enzymes, docking calculations were made, indicating that the residues His48 and Asp49 in the active site of these enzymes interacted powerfully with Har2 through hydrogen bonds. These data pointed to a possible anti-inflammatory activity of Har2 through sPLA(2) inhibition.en
dc.description.affiliationUniv Fed Ceara, Dept Fisiol & Farmacol, BR-60430270 Fortaleza, CE, Brazil
dc.description.affiliationUniversidade Federal de Pernambuco (UFPE), Dept Ciencias Farmaceut, BR-50740520 Recife, PE, Brazil
dc.description.affiliationUniv Fed Rio Grande do Norte, Dept Quim, BR-50078970 Natal, RN, Brazil
dc.description.affiliationUniv Fed Ceara, Ctr NE Aplicacao & Uso Ressonancia Magnet Nucl CE, BR-60455760 Fortaleza, CE, Brazil
dc.description.affiliationUniv Estadual Campinas, Inst Biomed, Dept Bioquim, BR-13082862 Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Unidade Sao Vicente, BR-11330900 Sao Vicente, SP, Brazil
dc.description.affiliationUniv Presbiteriana Mackenzie, Ctr Ciencias Biol & Saude, BR-01302970 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Unidade Sao Vicente, BR-11330900 Sao Vicente, SP, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico (FUNCAP)
dc.format.extent9
dc.identifierhttp://dx.doi.org/10.1155/2012/987517
dc.identifier.citationEvidence-based Complementary and Alternative Medicine. New York: Hindawi Publishing Corporation, p. 9, 2012.
dc.identifier.doi10.1155/2012/987517
dc.identifier.fileWOS000308300500001.pdf
dc.identifier.issn1741-427X
dc.identifier.urihttp://hdl.handle.net/11449/381
dc.identifier.wosWOS:000308300500001
dc.language.isoeng
dc.publisherHindawi Publishing Corporation
dc.relation.ispartofEvidence-based Complementary and Alternative Medicine
dc.relation.ispartofjcr2.064
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleInhibition of Neurotoxic Secretory Phospholipases A(2) Enzymatic, Edematogenic, and Myotoxic Activities by Harpalycin 2, an Isoflavone Isolated from Harpalyce brasiliana Benthen
dc.typeArtigo
dcterms.licensehttp://www.hindawi.com/journals/apm/apc/
dcterms.rightsHolderHindawi Publishing Corporation
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, São Vicentept

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Artigos - Ciências Biológicas - São Vicente