Determination of Cephalosporins by UHPLC-DAD Using Molecularly Imprinted Polymers

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dc.contributor.authorBaeza-Fonte, Alen N
dc.contributor.authorGarcés-Lobo, Idenia
dc.contributor.authorLuaces-Alberto, Markel D
dc.contributor.authorGonçalves, Luís Moreira
dc.contributor.authorSotomayor, Maria D P T [UNESP]
dc.contributor.authorValdés-González, Arístides C
dc.contributor.institutionUniversity of Havana
dc.contributor.institutionUniversidade Do Porto (FCUP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:17:51Z
dc.date.available2018-12-11T17:17:51Z
dc.date.issued2018-02-01
dc.description.abstractMolecularly imprinted polymers (MIPs) were synthesized for the determination of cephalosporins (cephazolin, ceftazidime, cefotaxime, ceftriaxone, cefepime and cephalexin) by ultra-high performance liquid chromatography with diode-array detection (UHPLC-DAD). After optimization, MIPs were synthesized using cephazolin as the template, methacrylic acid as the functional monomer, triethylenglycol dimethacrylate as the crosslinker, acetonitrile/dimethylsulfoxide as porogens and benzoyl peroxide as the radical initiator. Not only this is a novel route of MIP synthesis for cephalosporins, but also this choice of analytes is unique. Chromatographic separation was performed in a C 8 column using a binary gradient with trifluoroacetic acid 0.1% in water and acetonitrile. Linearity was assessed up to 100 μg mL -1 and linear correlation coefficients (r) were all higher than 0.99, limits of detection were within the range of 3-12 ng mL -1, and recoveries from 86 to 102% were obtained for concentrations between 0.05 and 1.0 μg mL -1.en
dc.description.affiliationUniversitary Laboratory for Characterization of the Structure of Substances Institute of Sciences and Technology of Materials University of Havana, Calle Zapata y G, s/n
dc.description.affiliationDepartment of Analytical Chemistry Faculty of Chemistry University of Havana, Calle Zapata y G, s/n
dc.description.affiliationREQUIMTE/LAQV Departamento de Química e Bioquímica Faculdade de Ciências Universidade Do Porto (FCUP), Rua do Campo Alegre, s/n
dc.description.affiliationDepartamento de Química Fundamental Instituto de Química Universidade de São Paulo (USP), Av. Prof. Lineu Prestes 748
dc.description.affiliationInstituto de Química UNESP-Univ Estadual Paulista Departamento de Química Analítica, Av. Prof. Francisco Degni, 55
dc.description.affiliationUnespInstituto de Química UNESP-Univ Estadual Paulista Departamento de Química Analítica, Av. Prof. Francisco Degni, 55
dc.format.extent187-193
dc.identifierhttp://dx.doi.org/10.1093/chromsci/bmx099
dc.identifier.citationJournal of Chromatographic Science, v. 56, n. 2, p. 187-193, 2018.
dc.identifier.doi10.1093/chromsci/bmx099
dc.identifier.file2-s2.0-85041660892.pdf
dc.identifier.issn0021-9665
dc.identifier.scopus2-s2.0-85041660892
dc.identifier.urihttp://hdl.handle.net/11449/175849
dc.language.isoeng
dc.relation.ispartofJournal of Chromatographic Science
dc.relation.ispartofsjr0,360
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.titleDetermination of Cephalosporins by UHPLC-DAD Using Molecularly Imprinted Polymersen
dc.typeArtigo

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