Transcription profiling of signal transduction-related genes in sugarcane tissues

Página do item simplificado
dc.contributor.authorPapini-Terzi, F. S.
dc.contributor.authorRocha, F. R.
dc.contributor.authorVencio, RZN
dc.contributor.authorOliveira, K. C.
dc.contributor.authorFelix, J. D.
dc.contributor.authorVicentini, R.
dc.contributor.authorRocha, C. D.
dc.contributor.authorSimoes, ACQ
dc.contributor.authorUlian, E. C.
dc.contributor.authorDi Mauro, SMZ
dc.contributor.authorDa Silva, A. M.
dc.contributor.authorPereira, CAD
dc.contributor.authorMenossi, M.
dc.contributor.authorSouza, G. M.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionCtr Tecnol Copersucar
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:17:32Z
dc.date.available2014-05-20T13:17:32Z
dc.date.issued2005-02-28
dc.description.abstractA collection of 237,954 sugarcane ESTs was examined in search of signal transduction genes. Over 3,500 components involved in several aspects of signal transduction, transcription, development, cell cycle, stress responses and pathogen interaction were compiled into the Sugarcane Signal Transduction (SUCAST) Catalogue. Sequence comparisons and protein domain analysis revealed 477 receptors, 510 protein kinases, 107 protein phosphatases, 75 small GTPases, 17 G-proteins, 114 calcium and inositol metabolism proteins, and over 600 transcription factors. The elements were distributed into 29 main categories subdivided into 409 sub-categories. Genes with no matches in the public databases and of unknown function were also catalogued. A cDNA microarray was constructed to profile individual variation of plants cultivated in the field and transcript abundance in six plant organs (flowers, roots, leaves, lateral buds, and 1(st) and 4(th) internodes). From 1280 distinct elements analyzed, 217 (17%) presented differential expression in two biological samples of at least one of the tissues tested. A total of 153 genes (12%) presented highly similar expression levels in all tissues. A virtual profile matrix was constructed and the expression profiles were validated by real-time PCR. The expression data presented can aid in assigning function for the sugarcane genes and be useful for promoter characterization of this and other economically important grasses.en
dc.description.affiliationUniv São Paulo, Inst Quim, Dept Bioquim, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Matemat & Estatist, Dept Estatist, BR-05508090 São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Inst Biol, Dept Genet & Evolucao, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationCtr Tecnol Copersucar, BR-13400970 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Ciências Agr & Vet Jaboticabal, Dept Tecnol, Ctr Estocagem Clones,BCCCtr, BR-14870000 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciências Agr & Vet Jaboticabal, Dept Tecnol, Ctr Estocagem Clones,BCCCtr, BR-14870000 São Paulo, Brazil
dc.format.extent27-38
dc.identifierhttp://dx.doi.org/10.1093/dnares/12.1.27
dc.identifier.citationDna Research. Oxford: Oxford Univ Press, v. 12, n. 1, p. 27-38, 2005.
dc.identifier.doi10.1093/dnares/12.1.27
dc.identifier.fileWOS000230919700003.pdf
dc.identifier.issn1340-2838
dc.identifier.urihttp://hdl.handle.net/11449/3966
dc.identifier.wosWOS:000230919700003
dc.language.isoeng
dc.publisherOxford University Press
dc.relation.ispartofDna Research
dc.relation.ispartofjcr5.415
dc.relation.ispartofsjr2,866
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectSaccharumpt
dc.subjectSugarcanept
dc.subjectmicroarraypt
dc.subjectsignal transductionpt
dc.subjecttranscriptomept
dc.titleTranscription profiling of signal transduction-related genes in sugarcane tissuesen
dc.typeArtigo
dcterms.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dcterms.rightsHolderOxford Univ Press
unesp.author.orcid0000-0001-9249-4922[11]
unesp.author.orcid0000-0001-6903-9913[14]
unesp.author.orcid0000-0002-0176-9722[8]
unesp.author.orcid0000-0002-9211-3787[13]
unesp.author.orcid0000-0001-6755-4153[10]
unesp.author.orcid0000-0002-8535-0901[4]
unesp.author.orcid0000-0003-0425-7877[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000230919700003.pdf
Tamanho:
691.53 KB
Formato:
Adobe Portable Document Format
Baixar
Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Tecnologia - FCAV