Manipulation of the periovulatory sex steroidal milieu affects endometrial but not luteal gene expression in early diestrus Nelore cows

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dc.contributor.authorMesquita, F. S.
dc.contributor.authorPugliesi, G.
dc.contributor.authorScolari, S. C.
dc.contributor.authorFranca, M. R.
dc.contributor.authorRamos, R. S.
dc.contributor.authorOliveira, M.
dc.contributor.authorPapa, P. C.
dc.contributor.authorBressan, F. F.
dc.contributor.authorMeirelles, F. V.
dc.contributor.authorSilva, L. A.
dc.contributor.authorNogueira, G. P. [UNESP]
dc.contributor.authorMeinbrive, C. M. B. [UNESP]
dc.contributor.authorBinelli, M.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-12-03T13:08:57Z
dc.date.available2014-12-03T13:08:57Z
dc.date.issued2014-04-01
dc.description.abstractIn beef cattle, the ability to conceive has been associated positively with size of the preovulatory follicle (POF). Proestrus estradiol and subsequent progesterone concentrations can regulate the endometrium to affect receptivity and fertility. The aim of the present study was to verify the effect of the size of the POF on luteal and endometrial gene expression during subsequent early diestrus in beef cattle. Eighty-three multiparous, nonlactating, presynchronized Nelore cows received a progesterone-releasing device and estradiol benzoate on Day-10 (D-10). Animals received cloprostenol (large follicle-large CL group; LF-LCL; N = 42) or not (small follicle-small CL group; SF-SCL; N = 41) on D-10. Progesterone devices were withdrawn and cloprostenol administered 42 to 60 hours (LF-LCL) or 30 to 36 hours (SF-SCL) before GnRH treatment (D0). Tissues were collected at slaughter on D7. The LF-LCL group had larger (P < 0.0001) POF (13.24 +/- 33 mm vs. 10.76 +/- 0.29 mm), greater (P < 0.0007) estradiol concentrations on D0 (2.94 +/- 0.28 pg/mL vs. 1.27 +/- 0.20 pg/mL), and greater (P < 0.01) progesterone concentrations on D7 (3.71 +/- 0.25 ng/mL vs. 2.62 +/- 0.26 ng/mL) compared with the SF-SCL group. Luteal gene expression of vascular endothelial growth factor A, kinase insert domain receptor, fms-related tyrosine kinase 1, steroidogenic acute regulatory protein, cytochrome P450, family 11, subfamily A, polypeptide 1, and hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid deltaisomerase 7 was similar between groups. Endometrial gene expression of oxytocin receptor and peptidase inhibitor 3, skin-derived was reduced, and estrogen receptor alpha 2, aldo-keto reductase family 1, member C4, and lipoprotein lipase expression was increased in LF-LCL versus SF-SCL Results support the hypothesis that the size of the POF alters the periovulatory endocrine milieu (i.e., proestrus estradiol and diestrus progesterone concentrations) and acts on the uterus to alter endometrial gene expression. It is proposed that the uterine environment and receptivity might also be modulated. Additionally, it is suggested that increased progesterone secretion of cows ovulating larger follicles is likely due to increased CL size rather than increased luteal expression of steroidogenic genes. @ 2014 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniv Sao Paulo, Fac Med Vet & Zootecnia, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Zootecnia & Engn Alimentos, Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Med Vet, Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Med Vet, Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Sao Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent861-869
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2013.12.022
dc.identifier.citationTheriogenology. New York: Elsevier Science Inc, v. 81, n. 6, p. 861-869, 2014.
dc.identifier.doi10.1016/j.theriogenology.2013.12.022
dc.identifier.issn0093-691X
dc.identifier.urihttp://hdl.handle.net/11449/111764
dc.identifier.wosWOS:000333438200012
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofTheriogenology
dc.relation.ispartofjcr2.136
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectProgesteroneen
dc.subjectEstradiolen
dc.subjectUterusen
dc.subjectCorpus luteumen
dc.subjectOvulatory follicleen
dc.titleManipulation of the periovulatory sex steroidal milieu affects endometrial but not luteal gene expression in early diestrus Nelore cowsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0002-5995-3944[13]
unesp.author.orcid0000-0002-9545-179X[10]
unesp.author.orcid0000-0002-3265-3271[7]
unesp.author.orcid0000-0003-0372-4920[9]
unesp.author.orcid0000-0002-9392-9526[5]
unesp.author.orcid0000-0001-9862-5874[8]

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