In vitro effects of antimicrobial agents on planktonic and biofilm forms of staphylococcus saprophyticusisolated from patients with urinary tract infections

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dc.contributor.authorMartins, Katheryne Benini [UNESP]
dc.contributor.authorFerreira, Adriano Martison [UNESP]
dc.contributor.authorPereira, Valéria Cataneli [UNESP]
dc.contributor.authorPinheiro, Luiza [UNESP]
dc.contributor.authorDe Oliveira, Adilson [UNESP]
dc.contributor.authorDe Lourdes Ribeiro De Souza Da Cunha, Maria [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2019-10-06T16:26:08Z
dc.date.available2019-10-06T16:26:08Z
dc.date.issued2019-01-01
dc.description.abstractBacterial biofilms play an important role in urinary tract infections (UTIs), being responsible for persistent infections that lead to recurrences and relapses. Staphylococcus saprophyticus is one of the main etiological agents of UTIs, however, little is known about biofilm production in this species and especially about its response to the antimicrobial agents used to treat UTIs when a biofilm is present. For this reason, the aim of this work was to evaluate the response of S. saprophyticus biofilms to five antimicrobial agents. Staphylococcus saprophyticus was evaluated for antimicrobial susceptibility in its planktonic form by means of minimum inhibitory concentration (MIC) and in biofilms by means of minimum inhibitory concentration in biofilm (MICB) against the following antimicrobial agents by the microdilution technique: Vancomycin, oxacillin, trimethoprim/sulfamethoxazole, ciprofloxacin, and norfloxacin. Of the 169 S. saprophyticus studied, 119 produced a biofilm as demonstrated by the polystyrene plate adherence method. Biofilm cells of S. saprophyticus exhibited a considerable increase in MICB when compared to the planktonic forms, with an increase of more than 32 times in the MICB of some drugs. Some isolates switched from the category of susceptible in the planktonic condition to resistant in the biofilm state. Statistical analysis of the results showed a significant increase in MICB (p < 0.0001) for all five drugs tested in the biofilm state compared to the planktonic form. Regarding determination of the minimum bactericidal concentration in biofilm (MBCB), there were isolates for which the minimum bactericidal concentration of all drugs was equal to or higher than the highest concentration tested.en
dc.description.affiliationDepartment of Microbiology and Immunology Institute of Biosciences UNESP - Universidade Estadual Paulista
dc.description.affiliationDepartment of Tropical Diseases Botucatu School of Medicine University Hospital UNESP - Universidade Estadual Paulista
dc.description.affiliationUnespDepartment of Microbiology and Immunology Institute of Biosciences UNESP - Universidade Estadual Paulista
dc.description.affiliationUnespDepartment of Tropical Diseases Botucatu School of Medicine University Hospital UNESP - Universidade Estadual Paulista
dc.identifierhttp://dx.doi.org/10.3389/fmicb.2019.00040
dc.identifier.citationFrontiers in Microbiology, v. 10, n. JAN, 2019.
dc.identifier.doi10.3389/fmicb.2019.00040
dc.identifier.issn1664-302X
dc.identifier.scopus2-s2.0-85064408553
dc.identifier.urihttp://hdl.handle.net/11449/188987
dc.language.isoeng
dc.relation.ispartofFrontiers in Microbiology
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAntimicrobial resistance
dc.subjectBiofilm
dc.subjectMBCB
dc.subjectMICB
dc.subjectStaphylococcus saprophyticus
dc.titleIn vitro effects of antimicrobial agents on planktonic and biofilm forms of staphylococcus saprophyticusisolated from patients with urinary tract infectionsen
dc.typeArtigo

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Artigos - Microbiologia e Imunologia - IBB
Artigos - Doenças Tropicais e Diagnósticos por Imagem - FMB