Atypical beta-adrenoceptor subtypes mediate relaxations of rabbit corpus cavernosum

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dc.contributor.authorTeixeira, C. E.
dc.contributor.authorBaracat, J. S.
dc.contributor.authorZanesco, A.
dc.contributor.authorAntunes, E.
dc.contributor.authorDe Nucci, G.
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:29:49Z
dc.date.available2014-05-20T15:29:49Z
dc.date.issued2004-05-01
dc.description.abstractThis study was performed to characterize the beta-adrenoceptor population in rabbit isolated corpus cavernosum (RbCC) by using nonselective and selective beta-adrenoceptor agonists and antagonists in functional assays. Metaproterenol, ritodrine, fenoterol, and 8-hydroxy-5-[(1R)-1-hydroxy-2-[N-[(1R)-2-(rho-methoxy-phenyl)1-methylethyl] amino] ethyl] carbostyril (TA 2005) (3-100 nmol each) dose dependently relaxed the RbCC preparations. These relaxations were markedly reduced by N-omega-nitro-L-arginine methyl ester (L-NAME; 10 muM) and 1H-[1,2,4]-oxadiazolo-[4,3,-a]quinoxalin-1-one (ODQ) (10 muM), whereas the adenylyl cyclase inhibitor SQ 22,536 [9-(2-tetrahydrofuryl)adenine] (10 muM) had no effect. In contrast, neither L-NAME nor ODQ affected the isoproterenol-induced RbCC relaxations, but SQ 22,536 abolished this response. Sildenafil (1 muM) significantly potentiated the relaxations induced by beta(2)-agonists without affecting the isoproterenol-evoked relaxations. Rolipram (10 muM) enhanced the relaxations elicited by isoproterenol but had no effect on those induced by the selective beta(2) agonists. Propranolol and (+/-)-1-[2,3-(dihydro-7-methyl-1H-inden-4-yl)oxy]-3-[(1-methylethyl)amino]-2-butanolhydrochloride (ICI 118,551) determined a rightward shift in the concentration-response curves to isoproterenol in a noncompetitive manner with a reduction of maximum response at the highest antagonist concentration, with the slope values significantly different from unity. Propranolol and ICI 118,551 had no effect on the relaxations elicited by fenoterol, TA 2005, metaproterenol, and ritodrine. Atenolol and 1-[2-((3-carbamoyl-4-hydroxy)phenoxy) ethylamino]-3-[4-(1-methyl-4-trifluoromethyl-2-imidazolyl)-phenoxy]2-propanol methanesulfonate (CGP 20712A) (0.1-10 muM) failed to affect the relaxations induced by all tested beta-adrenoceptor agonists. Our study revealed the existence of two atypical beta-adrenoceptors in the rabbit erectile tissue. Isoproterenol relaxes the rabbit cavernosal tissue by activating atypical beta-adrenoceptors coupled to adenylyl cyclase pathway, whereas the selective beta2-adrenoceptor agonists relax the RbCC tissue through another atypical beta-adrenoceptor subtype coupled to nitric oxide release from the sinusoidal endothelium.en
dc.description.affiliationState Univ Campinas, Fac Med Sci, Dept Pharmacol, Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Biosci Inst, Dept Educ Phys, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Biosci Inst, Dept Educ Phys, São Paulo, Brazil
dc.format.extent587-593
dc.identifierhttp://dx.doi.org/10.1124/jpet.103.062026
dc.identifier.citationJournal of Pharmacology and Experimental Therapeutics. Bethesda: Amer Soc Pharmacology Experimental Therapeutics, v. 309, n. 2, p. 587-593, 2004.
dc.identifier.doi10.1124/jpet.103.062026
dc.identifier.issn0022-3565
dc.identifier.lattes4472007237545596
dc.identifier.urihttp://hdl.handle.net/11449/39309
dc.identifier.wosWOS:000220972900020
dc.language.isoeng
dc.publisherAmer Soc Pharmacology Experimental Therapeutics
dc.relation.ispartofJournal of Pharmacology and Experimental Therapeutics
dc.relation.ispartofjcr3.706
dc.relation.ispartofsjr1,586
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleAtypical beta-adrenoceptor subtypes mediate relaxations of rabbit corpus cavernosumen
dc.typeArtigo
dcterms.licensehttp://jpet.aspetjournals.org/site/misc/terms.xhtml
dcterms.rightsHolderAmer Soc Pharmacology Experimental Therapeutics
unesp.author.lattes4472007237545596
unesp.author.orcid0000-0002-4346-7941[5]
unesp.author.orcid0000-0003-2201-8247[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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