Inflammation and apoptosis induced by mastoparan Polybia-MPII on skeletal muscle

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dc.contributor.authorRocha, Thalita
dc.contributor.authorSoares de Barros, Luciano Libardi
dc.contributor.authorFontana, Karina
dc.contributor.authorde Souza, Bibiana Monson [UNESP]
dc.contributor.authorPalma, Mario Sergio [UNESP]
dc.contributor.authorda Cruz-Hoefling, Maria Alice
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:55:08Z
dc.date.available2014-05-20T13:55:08Z
dc.date.issued2010-06-15
dc.description.abstractMastoparan firstly described as an inducer of mast cell granules exocytosis has been also related to many essential mechanisms of cell function. In skeletal muscle tissue the best characterization of mastoparan effect was induction of myonecrosis. We examined the ability of mastoparan Polybia-MPII from Polybia paulista wasp venom to induce apoptosis and inflammation in mouse tibial anterior muscle. The activation of caspase 3 and 9, the expression of INF-alpha, IFN-gamma, CD68 and CD163 proteins, specific of resident and migrant macrophages, respectively, were examined (3 h to 21 d). TUNEL-positive nuclei were found both in damaged and normal-looking muscle fibres, whereas the caspases, cytokines and macrophages proteins were only in damaged fibres. The caspase 3 and 9 expression and the immunolabelled areas of TNF-alpha and IFN-gamma were significantly higher compared to control. TUNEL-positive nuclei and TNF-alpha expression were also present in regenerating fibres. CD68 and CD163 signalize necrotic debris removal, release of chemo-attractants and cytokines which have been considered a pre-requisite for muscle regeneration. High levels of cytokines coincided with the intense muscle proteolysis by mastoparan (3-24 h) and the climax of regeneration (3 d) whereas cytokines decline corresponded to periods of tissue remodeling and intense fibre protein synthesis (7-21 d). We conclude that the mastoparan Polybia-mPII causes myonecrosis and apoptosis, the latter probably involving caspases signalling, corroborated by mitochondrial damage, and cytokines activation. (C) 2009 Elsevier Ltd. All rights reserved.en
dc.description.affiliationState Univ Campinas UNICAMP, Inst Biol, Dept Histol & Embryol, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationIBRC UNESP, CEIS, Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespIBRC UNESP, CEIS, Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdCNPq: 141336/2005-6
dc.description.sponsorshipIdFAPESP: 05/53625-1
dc.format.extent1213-1221
dc.identifierhttp://dx.doi.org/10.1016/j.toxicon.2009.12.005
dc.identifier.citationToxicon. Oxford: Pergamon-Elsevier B.V. Ltd, v. 55, n. 7, p. 1213-1221, 2010.
dc.identifier.doi10.1016/j.toxicon.2009.12.005
dc.identifier.issn0041-0101
dc.identifier.lattes2901888624506535
dc.identifier.urihttp://hdl.handle.net/11449/19721
dc.identifier.wosWOS:000277881900002
dc.language.isoeng
dc.publisherPergamon-Elsevier B.V. Ltd
dc.relation.ispartofToxicon
dc.relation.ispartofjcr2.352
dc.relation.ispartofsjr0,692
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCaspaseen
dc.subjectCytokinesen
dc.subjectMacrophageen
dc.subjectMyonecrosisen
dc.subjectSkeletal muscleen
dc.subjectTUNEL reactionen
dc.titleInflammation and apoptosis induced by mastoparan Polybia-MPII on skeletal muscleen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderPergamon-Elsevier B.V. Ltd
unesp.author.lattes2901888624506535
unesp.author.orcid0000-0002-2731-9586[1]
unesp.author.orcid0000-0002-7363-8211[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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