Multivariate optimization and validation of an analytical methodology by RP-HPLC for the determination of losartan potassium in capsules

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dc.contributor.authorBonfilio, Rudy
dc.contributor.authorTeixeira Tarley, Cesar Ricardo
dc.contributor.authorPereira, Gislaine Ribeiro
dc.contributor.authorSalgado, Hérida Regina Nunes [UNESP]
dc.contributor.authorde Araujo, Magali Benjamim
dc.contributor.institutionUniversidade Federal de Alfenas (UNIFAL)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2014-05-20T13:24:52Z
dc.date.available2014-05-20T13:24:52Z
dc.date.issued2009-11-15
dc.description.abstractThis paper describes the optimization and validation of an analytical methodology for the determination of losartan potassium in capsules by HPLC using 2(5-1) fractional factorial and Doehlert designs. This multivariate approach allows a considerable improvement in chromatographic performance using fewer experiments, without additional cost for columns or other equipment. The HPLC method utilized potassium phosphate buffer (pH 6.2: 58 mmol L(-1))-acetonitrile (65:35, v/v) as the mobile phase, pumped at a flow rate of 1.0 mL min(-1). An octylsilane column (100 mm x 4.6 mm W., 5 mu m) maintained at 35 degrees C was used as the stationary phase. UV detection was performed at 254 nm. The method was validated according to the ICH guidelines, showing accuracy, precision (intra-day relative standard deviation (R.S.D.) and inter-day R.S.D values <2.0%). selectivity, robustness and linearity (r=0.9998) over a concentration range from 30 to 70 mg L(-1) of losartan potassium. The limits of detection and quantification were 0.114 and 0.420 mg L(-1), respectively. The validated method may be used to quantify losartan potassium in capsules and to determine the stability of this drug. (C) 2009 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Fed Alfenas, Dept Farm, BR-37130000 Alfenas, MG, Brazil
dc.description.affiliationUniv Fed Alfenas, Dept Ciencias Exatas, BR-37130000 Alfenas, MG, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Farmaceut, Dept Farm & Medicamentos, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, UNICAMP, Inst Quim, Inst Nacl Ciência & Tecnol Bioanalit,Dept Quim An, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Farmaceut, Dept Farm & Medicamentos, BR-14801902 Araraquara, SP, Brazil
dc.description.sponsorshipCenter of Pharmaceutical Equivalence of the Federal University of Alfenas
dc.description.sponsorshipFederal University of Alfenas
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent236-241
dc.identifierhttp://dx.doi.org/10.1016/j.talanta.2009.06.060
dc.identifier.citationTalanta. Amsterdam: Elsevier B.V., v. 80, n. 1, p. 236-241, 2009.
dc.identifier.doi10.1016/j.talanta.2009.06.060
dc.identifier.issn0039-9140
dc.identifier.urihttp://hdl.handle.net/11449/7835
dc.identifier.wosWOS:000271055700036
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofTalanta
dc.relation.ispartofjcr4.244
dc.relation.ispartofsjr1,186
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectDoehlert designen
dc.subjectFractional factorial designen
dc.subjectLosartan potassiumen
dc.subjectReversed-phase chromatographyen
dc.subjectValidationen
dc.titleMultivariate optimization and validation of an analytical methodology by RP-HPLC for the determination of losartan potassium in capsulesen
dc.typeTrabalho apresentado em evento
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0002-0385-340X[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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