Effect of low-level laser therapy on odontoblast-like cells exposed to bleaching agent

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dc.contributor.authorLima, Adriano Fonseca
dc.contributor.authorRibeiro, Ana Paula Dias
dc.contributor.authorBasso, Fernanda Gonçalves
dc.contributor.authorBagnato, Vanderlei Salvador
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.authorMarchi, Giselle Maria
dc.contributor.authorde Souza Costa, Carlos Alberto [UNESP]
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Nove de Julho (UNINOVE)
dc.contributor.institutionUniversidade de Brasília (UnB)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:28:42Z
dc.date.available2014-05-27T11:28:42Z
dc.date.issued2013-03-25
dc.description.abstractThe aim of the present study was to evaluate the effect of low-level laser therapy (LLLT) on odontoblast-like MDPC-23 cells exposed to carbamide peroxide (CP 0.01 %-2.21 μg/mL of H2O2). The cells were seeded in sterile 24-well plates for 72 h. Eight groups were established according to the exposure or not to the bleaching agents and the laser energy doses tested (0, 4, 10, and 15 J/cm2). After exposing the cells to 0.01 % CP for 1 h, this bleaching solution was replaced by fresh culture medium. The cells were then irradiated (three sections) with a near-infrared diode laser (InGaAsP-780 ± 3 nm, 40 mW), with intervals of 24 h. The 0.01 % CP solution caused statistically significant reductions in cell metabolism and alkaline phosphate (ALP) activity when compared with those of the groups not exposed to the bleaching agent. The LLLT did not modulate cell metabolism; however, the dose of 4 J/cm2 increased the ALP activity. It was concluded that 0.01 % CP reduces the MDPC-23 cell metabolism and ALP activity. The LLLT in the parameters tested did not influence the cell metabolism of the cultured cells; nevertheless, the laser dose of 4 J/cm2 increases the ALP activity in groups both with and without exposure to the bleaching agent. © 2013 Springer-Verlag London.en
dc.description.affiliationDepartment of Restorative Dentistry, Piracicaba Dental School University of Campinas-UNICAMP, Avenida Limeira, 901, Piracicaba, 13404-903
dc.description.affiliationDepartment of Restorative Dentistry Nove de Julho University, Rua Vergueiro, 235, Sao Paulo, 01504-000
dc.description.affiliationDepartment of Operative Dentistry University of Brasília-UnB. Campus Universitário Darcy Ribeiro, 70910-900, Brasília
dc.description.affiliationDepartment of Pathology, Piracicaba Dental School University of Campinas-UNICAMP, Avenida Limeira, 901, Piracicaba, 13404-903
dc.description.affiliationPhysics Institute of São Carlos USP-University of Sao Paulo, C.P. 369, São Carlos, 13560-970
dc.description.affiliationDepartment of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry Univ. Estadual Paulista (UNESP), Rua Humaitá, 1680, Araraquara, 14801-903
dc.description.affiliationDepartment of Physiology and Pathology, Araraquara School of Dentistry Univ. Estadual Paulista (UNESP), Rua Humaitá, 1680, Araraquara, 14801-903
dc.description.affiliationUnespDepartment of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry Univ. Estadual Paulista (UNESP), Rua Humaitá, 1680, Araraquara, 14801-903
dc.description.affiliationUnespDepartment of Physiology and Pathology, Araraquara School of Dentistry Univ. Estadual Paulista (UNESP), Rua Humaitá, 1680, Araraquara, 14801-903
dc.format.extent1-6
dc.identifierhttp://dx.doi.org/10.1007/s10103-013-1309-2
dc.identifier.citationLasers in Medical Science, p. 1-6.
dc.identifier.doi10.1007/s10103-013-1309-2
dc.identifier.issn0268-8921
dc.identifier.issn1435-604X
dc.identifier.lattes4517484241515548
dc.identifier.scopus2-s2.0-84875142789
dc.identifier.urihttp://hdl.handle.net/11449/74865
dc.language.isoeng
dc.relation.ispartofLasers in Medical Science
dc.relation.ispartofjcr1.949
dc.relation.ispartofsjr0,713
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAlkaline phosphatase
dc.subjectBleaching treatment
dc.subjectLaser
dc.subjectLow-level laser therapy
dc.subjectOdontoblasts
dc.titleEffect of low-level laser therapy on odontoblast-like cells exposed to bleaching agenten
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights
unesp.author.lattes4517484241515548[7]
unesp.author.orcid0000-0002-7455-6867[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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