Cytogenetic and random amplified polymorphic DNA analysis of Leptodactylus species from rural and urban environments (Anura, Amphibia)

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dc.contributor.authorArruda, M. P. [UNESP]
dc.contributor.authorMorielle-Versute, E. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:23:32Z
dc.date.available2014-05-27T11:23:32Z
dc.date.issued2008-04-22
dc.description.abstractCytogenetic and random amplified polymorphic DNA analyses carried out in the species Leptodactylus podicipinus, L. ocellatus, L. labyrinthicus, and L. fuscus from rural and urban habitats of the northwest region of São Paulo State, Brazil, showed that the karyotypes (2n = 22), constitutive heterochromatin distribution and nucleolus organizer region (NOR) location did not differ between the populations from the two environments. The in situ hybridization with an rDNA probe confirmed the location of the NORs on chromosome 8 revealing an in tandem duplication of that region in one of the chromosomes of L. fuscus. DAPI showed that part of the C-band-positive heterochromatin is rich in AT, including that in the proximity the NORs in L. podicipinus and L. ocellatus. The molecular analyses showed that the two populations (urban and rural) of L. podicipinus and L. fuscus are similar from a genetic point of view. The urban and rural populations of species L. ocellatus and L. labyrinthicus showed differences in genetic structures, probably due to urbanization which interferes with the dispersion of those frogs. The marked differences observed between the two populations of L. ocellatus can be representing the cryptic condition of the species. Unweighted pair-group method of analysis and genetic distance analysis detected the genetic proximity between L. ocellatus and L. fuscus. The results indicate that there was no reduction in the genetic diversity in the populations from the urban environment; however, the survival of these frogs would not be guaranteed in the case of an increase in human impact especially for populations of L. labyrinthicus and L. ocellatus. ©FUNPEC-RP.en
dc.description.affiliationDepartamento de Zoologia e Botânica Instituto de Biociências, Letras e Ciências Exatas UNESP, São José do Rio Preto, SP
dc.description.affiliationUnespDepartamento de Zoologia e Botânica Instituto de Biociências, Letras e Ciências Exatas UNESP, São José do Rio Preto, SP
dc.format.extent161-176
dc.identifierhttp://www.geneticsmr.com//year2008/vol7-1/pdf/gmr402.pdf
dc.identifier.citationGenetics and Molecular Research, v. 7, n. 1, p. 161-176, 2008.
dc.identifier.file2-s2.0-42149163062.pdf
dc.identifier.issn1676-5680
dc.identifier.scopus2-s2.0-42149163062
dc.identifier.urihttp://hdl.handle.net/11449/70391
dc.language.isoeng
dc.relation.ispartofGenetics and Molecular Research
dc.relation.ispartofsjr0,439
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAmphibia
dc.subjectAnthropogenic
dc.subjectCytogenetic
dc.subjectGenetic diversity
dc.subjectLeptodactylidae
dc.subjectRandom amplified polymorphic DNA
dc.subjectribosome DNA
dc.subjectanimal cell
dc.subjectBrazil
dc.subjectchromosome 8
dc.subjectchromosome NOR
dc.subjectcytogenetics
dc.subjectDNA determination
dc.subjectDNA probe
dc.subjectfrog
dc.subjectgene duplication
dc.subjectgene location
dc.subjectgenetic variability
dc.subjectheterochromatin
dc.subjectin situ hybridization
dc.subjectkaryotype
dc.subjectLeptodactylus
dc.subjectLeptodactylus fuscus
dc.subjectLeptodactylus labyrinthicus
dc.subjectLeptodactylus ocellatus
dc.subjectLeptodactylus podicipinus
dc.subjectnonhuman
dc.subjectrandom amplified polymorphic DNA
dc.subjectrural area
dc.subjectspecies habitat
dc.subjectsurvival
dc.subjecturban area
dc.subjecturbanization
dc.subjectanimal
dc.subjectenvironment
dc.subjectfrogs and toads
dc.subjectgenetic polymorphism
dc.subjectgenetics
dc.subjecthuman
dc.subjectkaryotyping
dc.subjectpopulation genetics
dc.subjectrural population
dc.subjecturban population
dc.subjectAnura
dc.subjectAnimals
dc.subjectCytogenetics
dc.subjectEnvironment
dc.subjectGenetics, Population
dc.subjectHeterochromatin
dc.subjectHumans
dc.subjectKaryotyping
dc.subjectNucleolus Organizer Region
dc.subjectPolymorphism, Genetic
dc.subjectRural Population
dc.subjectUrban Population
dc.titleCytogenetic and random amplified polymorphic DNA analysis of Leptodactylus species from rural and urban environments (Anura, Amphibia)en
dc.typeArtigo
dcterms.licensehttp://www.geneticsmr.com/node/5
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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