Flavonoid Detection in Hydroethanolic Extract of Pouteria torta (Sapotaceae) Leaves by HPLC-DAD and the Determination of Its Mutagenic Activity

Página do item simplificado
dc.contributor.authorCosta, Daryne L. M. G. [UNESP]
dc.contributor.authorRinaldo, Daniel [UNESP]
dc.contributor.authorVaranda, Eliana A. [UNESP]
dc.contributor.authorSousa, Juliana F. de [UNESP]
dc.contributor.authorNasser, Ana L. M. [UNESP]
dc.contributor.authorSilva, Ana C. Z. [UNESP]
dc.contributor.authorBaldoqui, Debora C.
dc.contributor.authorVilegas, Wagner [UNESP]
dc.contributor.authorSantos, Lourdes Campaner dos [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionMato Grosso Fed Inst
dc.contributor.institutionUniversidade Estadual de Maringá (UEM)
dc.date.accessioned2015-03-18T15:54:42Z
dc.date.available2015-03-18T15:54:42Z
dc.date.issued2014-10-01
dc.description.abstractIt is well known that phytotherapy has grown in popularity in recent years. Because a drug cannot be administered without ensuring its effectiveness and safety, the standardization and regulation of phytotherapeutic drugs are required by the global market and governmental authorities. This article describes a simple and reliable high-performance liquid chromatography-diode array detection analysis method for the simultaneous detection of myricetin-3-O-beta-D-galactopyranoside, myricetin-3-O-alpha-L-arabinopyranoside, and myricetin-3-O-alpha-L-rhaminopyranoside present in the hydroethanolic extract (ethanol/H2O, 7:3, v/v) of Pouteria torta. The mutagenic activity of the extract was evaluated on Salmonella typhimurium and by an in vivo micronucleus test on the peripheral blood cells of Swiss mice. The linearity, sensitivity, selectivity, repeatability, accuracy, and precision of the assay were evaluated. The analytical curves were linear and exhibited good repeatability (with a deviation of less than 5%) and demonstrated good recovery (within the 83-107% range). The results demonstrate that the hydroethanolic extract exhibited a mutagenic activity in both assays, suggesting caution in the use of this plant in folk medicine.en
dc.description.affiliationSao Paulo State Univ, Inst Chem, Dept Organ Chem, UNESP, BR-14800900 Sao Paulo, Brazil
dc.description.affiliationMato Grosso Fed Inst, IFMT, Cuiaba, Mato Grosso, Brazil
dc.description.affiliationSao Paulo State Univ, Dept Chem, Fac Sci, UNESP, BR-14800900 Sao Paulo, Brazil
dc.description.affiliationSao Paulo State Univ, Fac Pharmaceut Sci, Dept Biol Sci, UNESP, BR-14800900 Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Maringa, Dept Chem, UEM, Maringa, Parana, Brazil
dc.description.affiliationSao Paulo State Univ, UNESP, BR-14800900 Sao Paulo, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Inst Chem, Dept Organ Chem, UNESP, BR-14800900 Sao Paulo, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Dept Chem, Fac Sci, UNESP, BR-14800900 Sao Paulo, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Fac Pharmaceut Sci, Dept Biol Sci, UNESP, BR-14800900 Sao Paulo, Brazil
dc.description.affiliationUnespSao Paulo State Univ, UNESP, BR-14800900 Sao Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent1103-1112
dc.identifierhttp://dx.doi.org/10.1089/jmf.2013.0116
dc.identifier.citationJournal Of Medicinal Food. New Rochelle: Mary Ann Liebert, Inc, v. 17, n. 10, p. 1103-1112, 2014.
dc.identifier.doi10.1089/jmf.2013.0116
dc.identifier.issn1096-620X
dc.identifier.lattes3538253640602977
dc.identifier.orcid0000-0003-3032-2556
dc.identifier.urihttp://hdl.handle.net/11449/116991
dc.identifier.wosWOS:000342563300008
dc.language.isoeng
dc.publisherMary Ann Liebert, Inc
dc.relation.ispartofJournal Of Medicinal Food
dc.relation.ispartofjcr1.954
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectmutagenicityen
dc.subjectliquid chromatographyen
dc.subjectPouteria tortaen
dc.subjectflavonoidsen
dc.subjectquantificationen
dc.titleFlavonoid Detection in Hydroethanolic Extract of Pouteria torta (Sapotaceae) Leaves by HPLC-DAD and the Determination of Its Mutagenic Activityen
dc.typeArtigo
dcterms.rightsHolderMary Ann Liebert, Inc
unesp.author.lattes3538253640602977
unesp.author.orcid0000-0001-5363-6481[2]
unesp.author.orcid0000-0003-3032-2556[8]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt

Arquivos

Coleções

Artigos - Química Orgânica - IQ