Canine parvovirus 2b in fecal samples of asymptomatic free-living South American coatis (Nasua nasua, Linnaeus, 1766)

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dc.contributor.authorSpera, Caroline Giuseppa
dc.contributor.authorLorenzetti, Elis
dc.contributor.authorPereira Lavorente, Fernanda Louise
dc.contributor.authorMarques, Gustavo de Calasans [UNESP]
dc.contributor.authorBisca, Jacqueline Muniz [UNESP]
dc.contributor.authorTeixeira, Carlos Roberto [UNESP]
dc.contributor.authorAlfieri, Amauri Alcindo
dc.contributor.authorAlfieri, Alice Fernandes
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniv Pitagoras Unopar
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2020-12-11T17:20:05Z
dc.date.available2020-12-11T17:20:05Z
dc.date.issued2020-05-14
dc.description.abstractCanine parvovirus type 2 (CPV-2) is classified into three subtypes (CPV-2a, CPV-2b, and CPV-2c) and is the main cause of enteritis and myocarditis in young domestic and wild animals. This study aimed to evaluate the presence of CPV-2 in the feces of asymptomatic free-living coatis from Garden Forest Reserve, Palmital city, SP, Brazil. Fecal samples from 21 coatis (both sexes, different ages, and different aspects of feces) were collected in August 2014 and March 2015. The nucleic acid extracted was submitted to a polymerase chain reaction (PCR) assay to amplify a fragment of the VP2 gene of CPV-2. Eight (38%) fecal samples were positive in the PCR assay and were confirmed by sequencing. The 7 nucleotide (nt) sequences analyzed showed 100% nt identity with the prototype strain of CPV-2b (CPV-39 strain). The analysis of the deduced amino acid (aa) sequence revealed the presence of the GAT codon (aa D-Asp) at position 426 of the VP2 viral protein (subtype 2b). This study describes for the first time the identification of CPV-2b in asymptomatic free-living coatis (Nasua nasua) and suggests that coatis are susceptible to Carnivore protoparvovirus 1 infection and are important as a reservoir and an asymptomatic carrier to other wild and domestic animal species.en
dc.description.affiliationUniv Estadual Londrina, Dept Vet Prevent Med, Lab Anim Virol, Celso Garcia Cid Rd,PR455 Km 380,POB 10011, BR-86057970 Londrina, Parana, Brazil
dc.description.affiliationUniv Estadual Londrina, Dept Vet Prevent Med, Mol Biol Unit, Multiuser Anim Hlth Lab, Celso Garcia Cid Rd,PR455 Km 380,POB 10011, BR-86057970 Londrina, Parana, Brazil
dc.description.affiliationUniv Pitagoras Unopar, Post Grad Program Anim Hlth & Prod, Arapongas, Parana, Brazil
dc.description.affiliationUniv Estadual Paulista, Vet Hosp, Ctr Med & Res Wild Anim, POB 560, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Vet Hosp, Ctr Med & Res Wild Anim, POB 560, BR-18618000 Botucatu, SP, Brazil
dc.format.extent1399-1403
dc.identifierhttp://dx.doi.org/10.1007/s42770-020-00293-2
dc.identifier.citationBrazilian Journal Of Microbiology. New York: Springer, v. 51, n. 3, p. 1399-1403, 2020.
dc.identifier.doi10.1007/s42770-020-00293-2
dc.identifier.issn1517-8382
dc.identifier.urihttp://hdl.handle.net/11449/197773
dc.identifier.wosWOS:000532651300001
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofBrazilian Journal Of Microbiology
dc.sourceWeb of Science
dc.subjectCoati
dc.subjectFeces
dc.subjectInfectious disease
dc.subjectMolecular detection
dc.subjectCPV-2b
dc.titleCanine parvovirus 2b in fecal samples of asymptomatic free-living South American coatis (Nasua nasua, Linnaeus, 1766)en
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes8560913137958850[6]
unesp.author.orcid0000-0001-6639-037X[6]

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