The effects of macromolecular and serum supplements and oxygen tension during bovine in vitro procedures on kinetics of oocyte maturation and embryo development

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dc.contributor.authorMingoti, Gisele Zoccal [UNESP]
dc.contributor.authorDias Caiado Castro, Viviane Sggobi [UNESP]
dc.contributor.authorMeo, Simone Cristina
dc.contributor.authorSa Barretto, Letcia Siqueira [UNESP]
dc.contributor.authorGarcia, Joaquim Mansano [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionBrazilian Agr Res Corp
dc.date.accessioned2014-05-20T13:15:22Z
dc.date.available2014-05-20T13:15:22Z
dc.date.issued2011-06-01
dc.description.abstractAiming to standardize in vitro production of bovine embryos and to obtain supplements to replace serum in culture media, this study evaluated the nuclear maturation kinetics and embryonic development in bovine after in vitro maturation (IVM) and culture (IVC) with several macromolecules (animal origin: bovine serum albumin (BSA), fetal calf serum (FCS); synthetic: polyvinyl alcohol (PVA), polyvinyl pyrrolidone (PVP), Ficoll, and Knockout) at two oxygen tensions (20% and 5% O(2)). Regarding nuclear kinetics, neither the presence of the expected stage (metaphase I, transition anaphase to telophase, and metaphase II) at each evaluation moment (6, 18, and 24 h after IVM, respectively) nor the accelerated polar body emission (at 18 h after IVM) related developmental competence to blastocyst stage when different supplements were compared. Independently of supplement, cleavage rates at 20% O(2) (61.6-79.2%) were higher than at 5% O(2) (38.9-58.7%). At 20% O(2), higher blastocyst and hatching rates, respectively, were obtained in treatments BSA, FCS, Knockout, and control group (IVM with FCS and IVC with BSA + FCS, 14.0-23.5% and 6.8-15.4%) in comparison to PVA, PVP, and Ficoll (0%). The same was observed at 5% O(2) for blastocyst rates with BSA, FCS, Knockout, and control (5.4-16.8%) and for hatching rates with BSA, FCS, and control (2.0-11.1%). We can conclude that producing bovine embryos at 20% O(2) during the entire IVP process resulted in higher developmental rates than at 5% O(2). In addition, while defined macromolecules PVA, PVP, and Ficoll were not suitable for embryonic development, the synthetic serum Knockout was able to replace serum and albumin for IVP in bovine at 20% O(2).en
dc.description.affiliationUniv Estadual Paulista, Fac Med Vet, BR-16050680 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Sch Vet Med, Dept Anim Hlth, BR-16050680 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Prevent Vet Med & Anim Reprod, Sch Agr & Vet Sci, BR-14884900 São Paulo, Brazil
dc.description.affiliationBrazilian Agr Res Corp, Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Cattle SE, BR-13560970 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Med Vet, BR-16050680 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Sch Vet Med, Dept Anim Hlth, BR-16050680 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Prevent Vet Med & Anim Reprod, Sch Agr & Vet Sci, BR-14884900 São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent361-367
dc.identifierhttp://dx.doi.org/10.1007/s11626-011-9400-0
dc.identifier.citationIn Vitro Cellular & Developmental Biology-animal. New York: Springer, v. 47, n. 5-6, p. 361-367, 2011.
dc.identifier.doi10.1007/s11626-011-9400-0
dc.identifier.issn1071-2690
dc.identifier.lattes4191951764334766
dc.identifier.orcid0000-0002-3059-4458
dc.identifier.urihttp://hdl.handle.net/11449/2541
dc.identifier.wosWOS:000291256200005
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofIn Vitro Cellular & Developmental Biology-animal
dc.relation.ispartofjcr1.447
dc.relation.ispartofsjr0,582
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCattleen
dc.subjectEmbryoen
dc.subjectIn vitro maturationen
dc.subjectMacromoleculeen
dc.subjectOxygenen
dc.titleThe effects of macromolecular and serum supplements and oxygen tension during bovine in vitro procedures on kinetics of oocyte maturation and embryo developmenten
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes2251116139872527
unesp.author.lattes4191951764334766
unesp.author.orcid0000-0002-3059-4458[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatubapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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Artigos - Apoio, Produção e Saúde Animal - FMVA
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