Paracoccidioides brasiliensis-gp43 used as paracoccidioidin

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dc.contributor.authorSaraiva, E. C O
dc.contributor.authorAltemani, A.
dc.contributor.authorFranco, M. F. [UNESP]
dc.contributor.authorUnterkircher, C. S. [UNESP]
dc.contributor.authorCamargo, Z. P.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:18:08Z
dc.date.available2014-05-27T11:18:08Z
dc.date.issued1996-12-01
dc.description.abstractA purified glycoprotein of 43 000 daltons from Paracoccidioides brasiliensis (gp43) was tested as paracoccidioidin in delayed-type hypersensitivity (DTH) tests in both experimental animals (guinea pig and mice) and patients with paracoccidioidomycosis (PCM). The gp43 paracoccidioidin was compared with the traditional Fava Netto antigen (AgFN). In guinea pigs, the intradermal injection of 2 μg of gp43 showed a similar response to those obtained with AgFN, showing in histological sections a population of lymphoid cells that participate in DTH. In mice, gp43 at a dose of 3.75μg showed positive DTH response. The use of gp43 as paracoccidioidin in humans showed that this molecule can be used to evaluate the DTH response in patients with PCM. Of 25 PCM patients studied, 48% were positive to gp43 while only 28% were positive to AgFN; 12 PCM patients were completely anergic to both antigens. Considering only those 13 PCM patients who were responsive to gp43 and/or to AgFN, 92.3% reacted against gp43 and 53.8% reacted against AgFN (P < 0.05). Gp43 skin test responses (13.67 ± 9.56 mm) were significantly larger than those obtained with AgFN (8.43 ±3.69 mm). Immunohistochemical study of the human skin showed a perivascular inflammatory response constituted predominantly by T lymphocytes, macrophages and polymorphonuclear leukocytes. © 1996 ISHAM.en
dc.description.affiliationUniversidade Federal de São Paulo Escola Paulista de Medidna Disciplina de Biologia Celular, Rua Botucatu, 862/8, 04023-062, São Paulo, SP
dc.description.affiliationDepartamento de Anatomia Patológica UNICAMP, Campinas, São Paulo, SP
dc.description.affiliationDepartamento de Anatomia Patológica Faculdade de Medidna de Botucatu UNESP, Botucatu, SP
dc.description.affiliationDepartamento de Patológia Faculdade de Odontologia de São José Dos Campos UNESP, SP
dc.description.affiliationUnespDepartamento de Anatomia Patológica Faculdade de Medidna de Botucatu UNESP, Botucatu, SP
dc.description.affiliationUnespDepartamento de Patológia Faculdade de Odontologia de São José Dos Campos UNESP, SP
dc.format.extent155-161
dc.identifierhttp://dx.doi.org/10.1080/02681219680000261
dc.identifier.citationJournal of Medical and Veterinary Mycology, v. 34, n. 3, p. 155-161, 1996.
dc.identifier.doi10.1080/02681219680000261
dc.identifier.issn0268-1218
dc.identifier.scopus2-s2.0-0029661850
dc.identifier.urihttp://hdl.handle.net/11449/64904
dc.identifier.wosWOS:A1996UN54900001
dc.language.isoeng
dc.relation.ispartofJournal of Medical and Veterinary Mycology
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectGp43
dc.subjectP. Brasiliensis
dc.subjectParacoccidioidin
dc.subjectParacoccidioidomycosis
dc.subjectfungus antigen
dc.subjectfungal protein
dc.subjectparacoccidioidin
dc.subjectanimal experiment
dc.subjectcontrolled study
dc.subjectdelayed hypersensitivity
dc.subjectguinea pig
dc.subjecthuman
dc.subjecthuman tissue
dc.subjectimmune response
dc.subjectimmunohistochemistry
dc.subjectinflammation
dc.subjectmouse
dc.subjectnonhuman
dc.subjectparacoccidioides brasiliensis
dc.subjectserum
dc.subjectskin
dc.subjectsouth american blastomycosis
dc.subjectadult
dc.subjectanimal
dc.subjectchemically induced disorder
dc.subjectcomparative study
dc.subjectimmunology
dc.subjectmale
dc.subjectmicrobiology
dc.subjectParacoccidioides
dc.subjectskin test
dc.subjectSouth American blastomycosis
dc.subjectAdult
dc.subjectAnimals
dc.subjectFungal Proteins
dc.subjectGuinea Pigs
dc.subjectHumans
dc.subjectHypersensitivity, Delayed
dc.subjectMale
dc.subjectMice
dc.subjectSkin Tests
dc.titleParacoccidioides brasiliensis-gp43 used as paracoccidioidinen
dc.typeArtigo
dcterms.licensehttp://informahealthcare.com/userimages/ContentEditor/1255620309227/Copyright_And_Permissions.pdf
unesp.author.orcid0000-0002-2971-7983[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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