Programmed cell death in the larval salivary glands of Apis mellifera (Hymenoptera, Apidae)

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dc.contributor.authorSilva-Zacarin, E. C. M.
dc.contributor.authorTomaino, G. A.
dc.contributor.authorBrocheto-Braga, M. R.
dc.contributor.authorTaboga, S. R.
dc.contributor.authorde Moraes, R. L. M. Silva
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:54:46Z
dc.date.available2014-05-20T13:54:46Z
dc.date.issued2007-03-01
dc.description.abstractThe morphological and histochemical features of degeneration in honeybee (Apis mellifera) salivary glands were investigated in 5th instar larvae and in the pre-pupal period. The distribution and activity patterns of acid phosphatase enzyme were also analysed. As a routine, the larval salivary glands were fixed and processed for light microscopy and transmission electron microscopy. Tissue sections were subsequently stained with haematoxylin-eosin, bromophenol blue, silver, or a variant of the critical electrolyte concentration (CEC) method. Ultrathin sections were contrasted with uranyl acetate and lead citrate. Glands were processed for the histochemical and cytochemical localization of acid phosphatase, as well as biochemical assay to detect its activity pattern. Acid phosphatase activity was histochemically detected in all the salivary glands analysed. The cytochemical results showed acid phosphatase in vesicles, Golgi apparatus and lysosomes during the secretory phase and, additionally, in autophagic structures and luminal secretion during the degenerative phase. These findings were in agreement with the biochemical assay. At the end of the 5th instar, the glandular cells had a vacuolated cytoplasm and pyknotic nuclei, and epithelial cells were shed into the glandular lumen. The transition phase from the 5th instar to the pre-pupal period was characterized by intense vacuolation of the basal cytoplasm and release of parts of the cytoplasm into the lumen by apical blebbing; these blebs contained cytoplasmic RNA, rough endoplasmic reticule and, occasionally, nuclear material. In the pre-pupal phase, the glandular epithelium showed progressive degeneration so that at the end of this phase only nuclei and remnants of the cytoplasm were observed. The nuclei were pyknotic, with peripheral chromatin and blebs. The gland remained in the haemolymph and was recycled during metamorphosis. The programmed cell death in this gland represented a morphological form intermediate between apoptosis and autophagy.en
dc.description.affiliationUniv Fed Sao Carlos, Sorocaba, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias Letras & Ciências Exatas, Dept Biol, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias Letras & Ciências Exatas, Dept Biol, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.format.extent309-328
dc.identifierhttp://dx.doi.org/10.1007/s12038-007-0031-2
dc.identifier.citationJournal of Biosciences. Bangalore: Indian Academy Sciences, v. 32, n. 2, p. 309-328, 2007.
dc.identifier.doi10.1007/s12038-007-0031-2
dc.identifier.issn0250-5991
dc.identifier.lattes1445259468526188
dc.identifier.orcid0000-0002-0970-4288
dc.identifier.urihttp://hdl.handle.net/11449/19624
dc.identifier.wosWOS:000245140800016
dc.language.isoeng
dc.publisherIndian Academy Sciences
dc.relation.ispartofJournal of Biosciences
dc.relation.ispartofjcr1.528
dc.relation.ispartofsjr0,651
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectdegenerationpt
dc.subjecthoneybeept
dc.subjectLarvapt
dc.subjectprepupapt
dc.subjectsilk glandpt
dc.titleProgrammed cell death in the larval salivary glands of Apis mellifera (Hymenoptera, Apidae)en
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights
dcterms.rightsHolderIndian Academy Sciences
unesp.author.lattes1445259468526188
unesp.author.orcid0000-0002-7982-0743[3]
unesp.author.orcid0000-0002-0970-4288[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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