Detection of enterotoxin and toxic shock syndrome toxin 1 genes in Staphylococcus, with emphasis on coagulase-negative staphylococci

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dc.contributor.authorCunha, Maria de Lourdes R. S. da [UNESP]
dc.contributor.authorCalsolari, Regina A. O. [UNESP]
dc.contributor.authorAraújo Jr., João Pessoa [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:22:27Z
dc.date.available2014-05-27T11:22:27Z
dc.date.issued2007-04-30
dc.description.abstractThe detection of staphylococcal enterotoxins is decisive for the confirmation of an outbreak and for the determination of the enterotoxigenicity of strains. Since the recognition of their antigenicity, a large number of serological methods for the detection of enterotoxins in food and culture media have been proposed. Since immunological methods require detectable amounts of toxin, molecular biology techniques represent important tools in the microbiology laboratory. In the present study, polymerase chain reaction (PCR) was used to identify genes responsible for the production of enterotoxins and toxic shock syndrome toxin 1 (TSST-1) in S. aureus and coagulase-negative staphylococci (CNS) isolated from patients and the results were compared with those obtained by the reverse passive latex agglutination (RPLA) assay. PCR detection of toxin genes revealed a higher percentage of toxigenic S. aureus strains (46.7%) than the RPLA method (38.3%). Analysis of the toxigenic profile of CNS strains showed that 26.7% of the isolates produced some type of toxin, and one or more toxin-specific genes were detected in 40% of the isolates. These results suggests the need for further studies in order to better characterize the pathogenic potential of CNS and indicate that attention should be paid to the toxigenic capacity of this group of microorganisms.en
dc.description.affiliationDepartamento de Microbiologia e Imunologia Instituto de Biociências Universidade Estadual Paulista, Botucatu, SP
dc.description.affiliationDepartamento de Microbiologia e Imunologia Instituto de Biociências Universidade Estadual Paulista, Caixa Postal 510, Botucatu, SP, CEP 18618-000
dc.description.affiliationUnespDepartamento de Microbiologia e Imunologia Instituto de Biociências Universidade Estadual Paulista, Botucatu, SP
dc.description.affiliationUnespDepartamento de Microbiologia e Imunologia Instituto de Biociências Universidade Estadual Paulista, Caixa Postal 510, Botucatu, SP, CEP 18618-000
dc.format.extent381-390
dc.identifierhttp://dx.doi.org/10.1111/j.1348-0421.2007.tb03925.x
dc.identifier.citationMicrobiology and Immunology, v. 51, n. 4, p. 381-390, 2007.
dc.identifier.doi10.1111/j.1348-0421.2007.tb03925.x
dc.identifier.issn0385-5600
dc.identifier.issn1348-0421
dc.identifier.scopus2-s2.0-34247375584
dc.identifier.urihttp://hdl.handle.net/11449/69631
dc.identifier.wosWOS:000245474900004
dc.language.isoeng
dc.relation.ispartofMicrobiology and Immunology
dc.relation.ispartofjcr1.335
dc.relation.ispartofsjr0,764
dc.relation.ispartofsjr0,764
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectCoagulase-negative staphylococci
dc.subjectEnterotoxins
dc.subjectPCR
dc.subjectTSST-1
dc.subjectcoagulase
dc.subjectenterotoxin
dc.subjecttoxic shock syndrome toxin 1
dc.subjectagglutination test
dc.subjectbacterial strain
dc.subjectgene identification
dc.subjectnonhuman
dc.subjectpolymerase chain reaction
dc.subjectStaphylococcus
dc.subjectStaphylococcus aureus
dc.subjectStaphylococcus epidermidis
dc.subjectStaphylococcus haemolyticus
dc.subjectStaphylococcus hominis
dc.subjectStaphylococcus lugdunensis
dc.subjectStaphylococcus simulans
dc.subjectstaphylococcus warneri
dc.subjectStaphylococcus xylosus
dc.subjecttoxin analysis
dc.subjectCoagulase
dc.subjectNucleic Acid Hybridization
dc.subjectPolymerase Chain Reaction
dc.subjectStaphylococcal Infections
dc.titleDetection of enterotoxin and toxic shock syndrome toxin 1 genes in Staphylococcus, with emphasis on coagulase-negative staphylococcien
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
unesp.author.orcid0000-0002-9153-1485[3]
unesp.author.orcid0000-0001-9079-2723[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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Artigos - Microbiologia e Imunologia - IBB