Immunohistochemical response in rats of beta-tricalcium phosphate (TCP) with or without BMP-2 in the production of collagen matrix critical defects

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dc.contributor.authorLuvizuto, Eloá Rodrigues [UNESP]
dc.contributor.authorde Oliveira, Júlio César Silva [UNESP]
dc.contributor.authorGomes-Ferreira, Pedro Henrique Silva [UNESP]
dc.contributor.authorPereira, Cassiano Costa Silva [UNESP]
dc.contributor.authorFaverani, Leonardo Perez [UNESP]
dc.contributor.authorAntoniali, Cristina [UNESP]
dc.contributor.authorOkamoto, Roberta [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:31:36Z
dc.date.available2018-12-11T17:31:36Z
dc.date.issued2017-04-01
dc.description.abstractThis study aimed to assess the biological response of BMP-2 (bone morphogenetic protein-2) in supplementation with β-tricalcium phosphate (TCP) as a carrier in the bone healing of surgical defects in rats’ calvaria. A critical-size defect (5 mm in diameter) was filled with β-TCP alone or added with that plus 5 mg of BMP-2 at 5, 15, and 30 postoperative days. Histomorphometric and immunohistochemical (osteocalcin, collagen type I, and metalloproteinase-9) analysis was performed to assess the features of bone healing. Histological behavior and collagen type I labeling showed increased formation of the collagen matrix, leading to a higher percentage of newly formed bone and biomaterial for tissue and more total mineralization of pure TCP when compared to the other groups. The supplementation with BMP-2 promoted faster TCP remodeling; however, there was no statistically significant difference for the bone formed in both groups (P > 0.05). Collagen-matrix formation and new bone formation reached maximum levels when the defects were filled with pure TCP, even exceeding the levels from BMP-2 supplementation.en
dc.description.affiliationDepartment of Surgery and Integrated Clinic Sao Paulo State University (UNESP) School of Dentistry
dc.description.affiliationDepartment of Basic Sciences Sao Paulo State University (UNESP) School of Dentistry
dc.description.affiliationUnespDepartment of Surgery and Integrated Clinic Sao Paulo State University (UNESP) School of Dentistry
dc.description.affiliationUnespDepartment of Basic Sciences Sao Paulo State University (UNESP) School of Dentistry
dc.format.extent302-308
dc.identifierhttp://dx.doi.org/10.1016/j.acthis.2017.02.006
dc.identifier.citationActa Histochemica, v. 119, n. 3, p. 302-308, 2017.
dc.identifier.doi10.1016/j.acthis.2017.02.006
dc.identifier.file2-s2.0-85014063447.pdf
dc.identifier.issn1618-0372
dc.identifier.issn0065-1281
dc.identifier.lattes1527011976590326
dc.identifier.scopus2-s2.0-85014063447
dc.identifier.urihttp://hdl.handle.net/11449/178675
dc.language.isoeng
dc.relation.ispartofActa Histochemica
dc.relation.ispartofsjr0,661
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBMP
dc.subjectCollagen matrix
dc.subjectHistologic evaluation
dc.subjectImmunohistochemistry
dc.titleImmunohistochemical response in rats of beta-tricalcium phosphate (TCP) with or without BMP-2 in the production of collagen matrix critical defectsen
dc.typeArtigo
unesp.author.lattes1527011976590326

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