Insulin-loaded polymeric mucoadhesive nanoparticles: Development, characterization and cytotoxicity evaluation

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dc.contributor.authorGatti, Tiago Henrique Honorato [UNESP]
dc.contributor.authorEloy, Josimar Oliveira [UNESP]
dc.contributor.authorFerreira, Leonardo Miziara Barboza [UNESP]
dc.contributor.authorDa Silva, Isabel Cristine [UNESP]
dc.contributor.authorPavan, Fernando Rogério [UNESP]
dc.contributor.authorGremião, Maria Palmira Daflon [UNESP]
dc.contributor.authorChorilli, Marlus [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:20:51Z
dc.date.available2018-12-11T17:20:51Z
dc.date.issued2018-01-01
dc.description.abstractMucoadhesive nanoparticles are particularly interesting for delivery through nasal or pulmonary routes, as an approach to overcome the mucociliary clearance. Moreover, these nanoparticles are attractive for peptide and protein delivery, particularly for insulin to treat diabetes, as an alternative to conventional parenteral administration. Thus, chitosan, a cationic mucoadhesive polysaccharide found in shells of crustaceans, and the negatively-charged dextran sulfate are able to form nanoparticles through ionic condensation, representing a potential insulin carrier. Herein, chitosan/dextran sulfate nanoparticles at various ratios were prepared for insulin loading. Formulations were characterized for particle size, zeta potential, encapsulation efficiency, scanning electron microscopy, differential scanning calorimetry, and in vitro drug release. Moreover, the interaction with mucin and the cytotoxicity against a lung cell line were studied, which altogether have not been addressed before. Results evidenced that a proper selection of polyelectrolytes is necessary for smaller particle size formation and also the composition and zeta potential impact encapsulation efficiency, which is benefited by the positive charge of chitosan. Insulin remained stable after encapsulation as evidenced by calorimetric assays, and was released in a sustained manner in the first 10 h. Positively-charged nanoparticles based on chitosan/dextran-sulfate at the ratio of 6:4 successfully interacted with mucin, which is a prerequisite for delivery to mucus-containing tissues. Finally, insulin-loaded nanoparticles displayed no cytotoxicity effect against lung cells at tested concentrations, suggesting the potential for further in vivo studies.en
dc.description.affiliationSchool of Pharmaceutical Sciences UNESP São Paulo State University Department of Drugs and Medicines, Campus Araraquara
dc.description.affiliationSchool of Pharmaceutical Sciences UNESP São Paulo State University Department of Biological Sciences, Campus Araraquara
dc.description.affiliationUnespSchool of Pharmaceutical Sciences UNESP São Paulo State University Department of Drugs and Medicines, Campus Araraquara
dc.description.affiliationUnespSchool of Pharmaceutical Sciences UNESP São Paulo State University Department of Biological Sciences, Campus Araraquara
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFibrolamellar Cancer Foundation
dc.description.sponsorshipUniversidade Estadual Paulista
dc.description.sponsorshipIdFAPESP: #2014/24180-0
dc.identifierhttp://dx.doi.org/10.1590/s2175-97902018000117314
dc.identifier.citationBrazilian Journal of Pharmaceutical Sciences, v. 54, n. 1, 2018.
dc.identifier.doi10.1590/s2175-97902018000117314
dc.identifier.fileS1984-82502018000100623.pdf
dc.identifier.issn2175-9790
dc.identifier.issn1984-8250
dc.identifier.lattes9129780536724256
dc.identifier.scieloS1984-82502018000100623
dc.identifier.scopus2-s2.0-85048537433
dc.identifier.urihttp://hdl.handle.net/11449/176454
dc.language.isoeng
dc.relation.ispartofBrazilian Journal of Pharmaceutical Sciences
dc.relation.ispartofsjr0,214
dc.relation.ispartofsjr0,214
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectChitosan
dc.subjectDextran-sulfate
dc.subjectInsulin
dc.subjectMucoadhesion
dc.subjectNanoparticles
dc.titleInsulin-loaded polymeric mucoadhesive nanoparticles: Development, characterization and cytotoxicity evaluationen
dc.typeArtigo
unesp.author.lattes9129780536724256

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