Identification of the endangered small red brocket deer (Mazama bororo) using noninvasive genetic techniques (Mammalia; Cervidae)

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dc.contributor.authorGonzalez, Susana
dc.contributor.authorMaldonado, Jesus E.
dc.contributor.authorOrtega, Jorge
dc.contributor.authorTalarico, Angela Cristina [UNESP]
dc.contributor.authorBidegaray-Batista, Leticia
dc.contributor.authorGarcia, José Fernando [UNESP]
dc.contributor.authorBarbanti Duarte, Jose Mauricio [UNESP]
dc.contributor.institutionIIBCE
dc.contributor.institutionSmithsonian Inst
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.date.accessioned2014-05-20T13:18:35Z
dc.date.available2014-05-20T13:18:35Z
dc.date.issued2009-05-01
dc.description.abstractThe small red brocket deer Mazama bororo is one of the most endangered deer in the Neotropics. The great morphological similarities with three other sympatric brocket deer species, coupled with the fact that they inhabit densely forested habitats complicate detection and prevent the use of traditional methodologies for accurate identification of species. The ability to determine the presence of this endangered species in an area is crucial for estimating its distribution range, and is critical for establishing conservation management strategies. Here we describe a fast and reliable noninvasive genetic method for species identification of Mazama species from faeces. We designed a primer set that amplifies a short 224-bp fragment of the cytochrome b and demonstrate its effectiveness in successful amplification of DNA isolated from both tissue and faecal samples. This fragment contains a BSTNI/ECORII digestion site that is unique to the endangered M. bororo. The digested polymerase chain reaction products yielded a 160-bp fragment that is clearly visible in a 2% agarose gel. Two other diagnostic sites were identified to differentiate the other three sympatric species, SspI (M. gouazoubira) and AflIII (M. americana, and M. nana).en
dc.description.affiliationIIBCE, Dept Genet, Fac Ciencias UdelaR, Unidad Genet Conservac, Montevideo, Uruguay
dc.description.affiliationSmithsonian Inst, Ctr Conservat & Evolutionary Genet, NZP NMNH, Washington, DC 20008 USA
dc.description.affiliationEscuela Nacl Ciencias Biol, Lab Ictiol & Limnol, Inst Politecn Nacl, Col Santo Tomas 11340, Mexico
dc.description.affiliationSão Paulo State Univ, NUPECCE, Dept Zootecnia, FCAV UNESP, BR-14884900 Jaboticabal, SP, Brazil
dc.description.affiliationUniversidade Federal de Pernambuco (UFPE), Ctr Acad Vitoria, BR-55608680 Vitoria de Santo Antao, PE, Brazil
dc.description.affiliationUnespSão Paulo State Univ, NUPECCE, Dept Zootecnia, FCAV UNESP, BR-14884900 Jaboticabal, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipPROBIO/MMA
dc.description.sponsorshipCSIC-UdelaR
dc.description.sponsorshipPEDECIBA
dc.description.sponsorshipSmithsonian Center for Conservation and Evolutionary Genetics
dc.description.sponsorshipFriends of the National Zoo and Wildlife Trust Alliance
dc.format.extent754-758
dc.identifierhttp://dx.doi.org/10.1111/j.1755-0998.2008.02390.x
dc.identifier.citationMolecular Ecology Resources. Malden: Wiley-blackwell Publishing, Inc, v. 9, n. 3, p. 754-758, 2009.
dc.identifier.doi10.1111/j.1755-0998.2008.02390.x
dc.identifier.issn1755-098X
dc.identifier.urihttp://hdl.handle.net/11449/4613
dc.identifier.wosWOS:000265897200012
dc.language.isoeng
dc.publisherWiley-Blackwell Publishing, Inc
dc.relation.ispartofMolecular Ecology Resources
dc.relation.ispartofjcr7.059
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCryptic speciesen
dc.subjectMazamaen
dc.subjectnon invasive samplingen
dc.subjectsmall red brocket deeren
dc.subjectspecies identificationen
dc.titleIdentification of the endangered small red brocket deer (Mazama bororo) using noninvasive genetic techniques (Mammalia; Cervidae)en
dc.typeArtigo
dcterms.licensehttp://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1601-0825/homepage/ForAuthors.html
dcterms.rightsHolderWiley-blackwell Publishing, Inc
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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Artigos - Zootecnia - FCAV