Distribution of Xylella fastidiosa in citrus rootstocks and transmission of citrus variegated chlorosis between sweet orange plants through natural root grafts

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dc.contributor.authorHe, C. X. [UNESP]
dc.contributor.authorLi, W. B.
dc.contributor.authorAyres, A. J.
dc.contributor.authorHartung, J. S.
dc.contributor.authorMiranda, V. S.
dc.contributor.authorTeixeira, D. C.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFundecitrus
dc.contributor.institutionAgricultural Research Service (ARS)
dc.date.accessioned2014-05-27T11:19:54Z
dc.date.available2014-05-27T11:19:54Z
dc.date.issued2000-06-01
dc.description.abstractTo study translocation of Xylella fastidiosa to citrus rootstocks, budsticks from citrus variegated chlorosis (CVC)-affected cv. Pera sweet orange (Citrus sinenesis (L.) Osb.) were top grafted on 15 citrus rootstocks. Disease symptoms were conspicuous 3 months later on all 15 rootstocks tested. The presence of X. fastidiosa was confirmed by light microscopy, double-antibody sandwich enzyme-linked immunosorbent assays, and polymerase chain reaction in rootlets and main roots of CVC-symptomatic Pera sweet orange in 11 of the 15 rootstocks tested. These results suggest that bacterial translocation from the aerial plant parts to the root system occurs but is not essential for X. fastidiosa to induce symptoms in the aerial parts. Bacterial translocation to the roots was not correlated with CVC leaf-symptom severity in the Pera scion. To determine if CVC disease could be transmitted by natural root grafts, two matched seedlings of each of four sweet orange cultivars (Pera, Natal, Valencia, and Caipira) were transplanted into single pots. One seedling rootstock of each pair was inoculated by top grafting with a CVC-contaminated budstick while the other seedling rootstock was cut but not graft inoculated. Transmission of X. fastidiosa from an inoculated plant to a noninoculated plant sharing the same pot was observed in all four sweet orange cultivars tested. Transmission was confirmed by observation of natural roots grafts between the two plants, presence of X. fastidiosa in the root grafts, and disease development in the uninoculated plants. This is the first report of transmission of CVC disease through natural root grafts.en
dc.description.affiliationDepartment of Technology UNESP, Jaboticabal, SP
dc.description.affiliationFUNDECITRUS, Araraquara, SP
dc.description.affiliationUSDA-ARS, Beltsville, MD 20705-2350
dc.description.affiliationUnespDepartment of Technology UNESP, Jaboticabal, SP
dc.format.extent622-626
dc.identifierhttp://dx.doi.org/10.1094/PDIS.2000.84.6.622
dc.identifier.citationPlant Disease, v. 84, n. 6, p. 622-626, 2000.
dc.identifier.doi10.1094/PDIS.2000.84.6.622
dc.identifier.issn0191-2917
dc.identifier.scopus2-s2.0-0034030155
dc.identifier.urihttp://hdl.handle.net/11449/66164
dc.identifier.wosWOS:000087161400004
dc.language.isoeng
dc.relation.ispartofPlant Disease
dc.relation.ispartofjcr2.941
dc.relation.ispartofsjr0,575
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCitrus
dc.subjectPlant-pathogenic bacteria
dc.subjectXylem
dc.subjectXylem-inhabiting bacteria
dc.subjectbacterial infection
dc.subjectbacterial translocation
dc.subjectcitrus variegated chlorosis
dc.subjectcultivar
dc.subjectdisease transmission
dc.subjectenzyme linked immunosorbent assay
dc.subjectgraft hybridization
dc.subjectmicroscopy
dc.subjectorange
dc.subjectpathogenesis
dc.subjectpolymerase chain reaction
dc.subjectrootstock
dc.subjectCitrus sinensis
dc.subjectXylella fastidiosa
dc.titleDistribution of Xylella fastidiosa in citrus rootstocks and transmission of citrus variegated chlorosis between sweet orange plants through natural root graftsen
dc.typeArtigo
dcterms.licensehttp://apsjournals.apsnet.org/page/terms.jsp
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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