Effects of Ginkgo biloba on chemically-induced mammary tumors in rats receiving tamoxifen

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dc.contributor.authorDias, Marcos Correa [UNESP]
dc.contributor.authorFurtado, Kelly Silva [UNESP]
dc.contributor.authorRodrigues, Maria Aparecida Marchesan [UNESP]
dc.contributor.authorBarbisan, Luis Fernando [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:29:04Z
dc.date.available2014-05-27T11:29:04Z
dc.date.issued2013-05-01
dc.description.abstractBackground: Ginkgo biloba extract (GbE) is used extensively by breast cancer patients undergoing treatment with Tamoxifen (TAM). Thus, the present study investigated the effects of GbE in female Sprague-Dawley (SD) rats bearing chemically-induced mammary tumors and receiving TAM.Methods: Animals bearing mammary tumors (≥1 cm in diameter) were divided into four groups: TAM [10 mg/kg, intragastrically (i.g.)], TAM plus GbE [50 and 100 mg/kg, intraperitoneally (i.p.)] or an untreated control group. After 4 weeks, the therapeutic efficacy of the different treatments was evaluated by measuring the tumor volume (cm3) and the proportions of each tumor that were alive, necrotic or degenerative (mm2). In addition, labeling indexes (LI%) were calculated for cell proliferation (PCNA LI%) and apoptosis (cleaved caspase-3 LI%), expression of estrogen receptor-alpha (ER-α) and p63 biomarkers.Results: Overall, the tumor volume and the PCNA LI% within live tumor areas were reduced by 83% and 99%, respectively, in all TAM-treated groups when compared to the untreated control group. GbE treatment (100 mg/kg) reduced the proportions of live (24.8%) and necrotic areas (2.9%) (p = 0.046 and p = 0.038, respectively) and significantly increased the proportion of degenerative areas (72.9%) (p = 0.004) in mammary tumors when compared to the group treated only with TAM. The expression of ER-α, p63 and cleaved caspase-3 in live tumor tissues was not modified by GbE treatment.Conclusions: Co-treatment with 100 mg/kg GbE presented a slightly beneficial effect on the therapeutic efficacy of TAM in female SD rats bearing mammary tumors. © 2013 Dias et al.; licensee BioMed Central Ltd.en
dc.description.affiliationPost-Graduation Program in Pathology School of Medicine Universidade Estadual Paulista (UNESP), Botucatu, SP 18618-970
dc.description.affiliationDepartment of Pathology School of Medicine Universidade Estadual Paulista (UNESP), Botucatu, SP 18618-970
dc.description.affiliationDepartment of Morphology Universidade Estadual Paulista (UNESP) Institute of Biosciences, Botucatu, SP 18618-970
dc.description.affiliationUnespPost-Graduation Program in Pathology School of Medicine Universidade Estadual Paulista (UNESP), Botucatu, SP 18618-970
dc.description.affiliationUnespDepartment of Pathology School of Medicine Universidade Estadual Paulista (UNESP), Botucatu, SP 18618-970
dc.description.affiliationUnespDepartment of Morphology Universidade Estadual Paulista (UNESP) Institute of Biosciences, Botucatu, SP 18618-970
dc.identifierhttp://dx.doi.org/10.1186/1472-6882-13-93
dc.identifier.citationBMC Complementary and Alternative Medicine, v. 13.
dc.identifier.doi10.1186/1472-6882-13-93
dc.identifier.file2-s2.0-84876796984.pdf
dc.identifier.issn1472-6882
dc.identifier.lattes3278528112652257
dc.identifier.scopus2-s2.0-84876796984
dc.identifier.urihttp://hdl.handle.net/11449/75272
dc.identifier.wosWOS:000319153300001
dc.language.isoeng
dc.relation.ispartofBMC Complementary and Alternative Medicine
dc.relation.ispartofjcr2.109
dc.relation.ispartofsjr0,858
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectComplementary and alternative medicine
dc.subjectMammary carcinogenesis
dc.subjectRat
dc.subjectSelective estrogen receptor modulator
dc.subjectTamoxifen
dc.subjectcaspase 3
dc.subjectestrogen receptor alpha
dc.subjectGinkgo biloba extract
dc.subjectprotein p63
dc.subjecttamoxifen
dc.subjecttamoxifen citrate
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectantineoplastic activity
dc.subjectapoptosis
dc.subjectbreast tumor
dc.subjectcell proliferation
dc.subjectcontrolled study
dc.subjectfemale
dc.subjectGinkgo biloba
dc.subjectnonhuman
dc.subjectprotein cleavage
dc.subjectprotein expression
dc.subjectrat
dc.subjecttumor volume
dc.subjectAnimals
dc.subjectAntineoplastic Agents
dc.subjectApoptosis
dc.subjectBreast Neoplasms
dc.subjectCell Proliferation
dc.subjectDrug Therapy, Combination
dc.subjectEstrogen Receptor alpha
dc.subjectFemale
dc.subjectGene Expression Regulation, Neoplastic
dc.subjectHumans
dc.subjectMammary Neoplasms, Animal
dc.subjectPlant Extracts
dc.subjectRats
dc.subjectRats, Sprague-Dawley
dc.titleEffects of Ginkgo biloba on chemically-induced mammary tumors in rats receiving tamoxifenen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
unesp.author.lattes3278528112652257
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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