Differential gene expression by Paracoccidioides brasiliensis in host interaction conditions: Representational difference analysis identifies candidate genes associated with fungal pathogenesis

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dc.contributor.authorBailao, Alexandre Melo
dc.contributor.authorSchrank, Augusto
dc.contributor.authorBorges, Clayton Luiz
dc.contributor.authorDutra, Valeria
dc.contributor.authorWalquiria Ines Molinari-Madlum, Eugenia Emilia
dc.contributor.authorSoares Felipe, Maria Sueli
dc.contributor.authorMendes-Giannini, Maria José Soares [UNESP]
dc.contributor.authorMartins, Wellington Santos
dc.contributor.authorPereira, Maristela
dc.contributor.authorde Almeida Soares, Celia Maria
dc.contributor.institutionUniversidade Federal de Goiás (UFG)
dc.contributor.institutionUniversidade Federal do Rio Grande do Sul (UFRGS)
dc.contributor.institutionUniversidade de Brasília (UnB)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Catholica Goias
dc.date.accessioned2014-05-20T15:29:53Z
dc.date.available2014-05-20T15:29:53Z
dc.date.issued2006-10-01
dc.description.abstractParacoccidioides brasiliensis causes infection by the host inhalation of airborne propagules of the mycelia phase of the fungus. These particles reach the lungs, and disseminate to virtually all organs. Here we describe the identification of differentially expressed genes in studies of host-fungus interaction. We analyzed two cDNA populations of P. brasiliensis, one obtained from infected animals and the other an admixture of fungus and human blood thus mimicking the hematologic events of the fungal dissemination. Our analysis identified transcripts differentially expressed. Genes related to iron acquisition, melanin synthesis and cell defense were specially upregulated in the mouse model of infection. The upregulated transcripts of yeast cells during incubation with human blood were those predominantly related to cell wall remodeling/synthesis. The expression pattern of genes was independently confirmed in host conditions, revealing their potential role in the infection process. This work can facilitate functional studies of novel regulated genes that may be important for the survival and growth strategies of P. brasiliensis in humans. (c) 2006 Elsevier Masson SAS. All rights reserved.en
dc.description.affiliationUniv Fed Goias, Inst Ciências Biol, Mol Biol Lab, BR-74001970 Goiania, Go, Brazil
dc.description.affiliationUniv Fed Rio Grande Sul, Ctr Biotecnol, Porto Alegre, RS, Brazil
dc.description.affiliationUniv Fed Goias, Inst Patol Trop & Saúde Publ, Goias, Brazil
dc.description.affiliationUniv Brasilia, Inst Ciências Biol, Brasilia, DF, Brazil
dc.description.affiliationUniv Estadual Julio Mesquita Filho, Fac Ciências Farmaceut, São Paulo, Brazil
dc.description.affiliationUniv Catholica Goias, Dept Comp, Goiania, Go, Brazil
dc.description.affiliationUnespUniv Estadual Julio Mesquita Filho, Fac Ciências Farmaceut, São Paulo, Brazil
dc.format.extent2686-2697
dc.identifierhttp://dx.doi.org/10.1016/j.micinf.2006.07.019
dc.identifier.citationMicrobes and Infection. Amsterdam: Elsevier B.V., v. 8, n. 12-13, p. 2686-2697, 2006.
dc.identifier.doi10.1016/j.micinf.2006.07.019
dc.identifier.issn1286-4579
dc.identifier.orcid0000-0002-8059-0826
dc.identifier.urihttp://hdl.handle.net/11449/39363
dc.identifier.wosWOS:000242671200003
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMicrobes and Infection
dc.relation.ispartofjcr2.924
dc.relation.ispartofsjr1,205
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectParacoccidioides brasiliensispt
dc.subjectrepresentational difference analysispt
dc.subjectinfectionpt
dc.titleDifferential gene expression by Paracoccidioides brasiliensis in host interaction conditions: Representational difference analysis identifies candidate genes associated with fungal pathogenesisen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0002-8059-0826[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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Artigos - Análises Clínicas - FCFAR