Proliferation and apoptotic rates and increased frequency of p63-positive cells in the prostate acinar epithelium of alloxan-induced diabetic rats

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dc.contributor.authorArcolino, Fanny Oliveira [UNESP]
dc.contributor.authorRibeiro, Daniele Lisboa [UNESP]
dc.contributor.authorGobbo, Marina Guimaraes [UNESP]
dc.contributor.authorTaboga, Sebastiao Roberto [UNESP]
dc.contributor.authorGoes, Rejane Maira [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T14:01:07Z
dc.date.available2014-05-20T14:01:07Z
dc.date.issued2010-04-01
dc.description.abstractP>The effects of experimental type 1 diabetes were investigated in the acinar epithelium of rat ventral prostate, focusing on the rates of cell proliferation and the frequency of apoptosis and p63-positive cells. Type 1 diabetes was induced in adult male Wistar rats by a single alloxan administration (42 mg/kg b.w.) and its effects were analysed for 1 week and 3 months after the establishment of the disease. A group of diabetic rats was treated daily with 5 IU of insulin during 1 week after diabetes had been diagnosed. Immunocytochemical methods for the localization of cell proliferation antigen (PCNA), androgen receptor (AR) and p63 protein were carried out, and apoptotic cells were identified by TUNEL essay. In diabetic rats, testosterone levels reduced drastically after 1 week and in a lower degree after 3 months. In short-term diabetic rats, cell proliferation decreased, and in medium-term, epithelial apoptotic rates increased. In both periods after the onset of diabetes, the frequency of p63-positive cells doubled. Insulin treatment was effective in preventing testosterone decrease, p63-positive cell increase and apoptotic rates, but did not interfere in cell proliferation. This investigation shows that, soon after diabetes onset, there are important modifications in cell proliferation within the acinar prostatic epithelium, and in longer term, there is a marked impact on kinetics of differentiation and cell death, which may initially be attributable to an androgenic fall, but is probably also because of other factors related to diabetes, as changes are considerably different from those resulting from castration.en
dc.description.affiliationSão Paulo State Univ, Inst Biosci Letters & Exact Sci, Dept Biol, BR-15054000 São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Inst Biosci Letters & Exact Sci, Dept Biol, BR-15054000 São Paulo, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 06/07008-3
dc.format.extent144-154
dc.identifierhttp://dx.doi.org/10.1111/j.1365-2613.2009.00696.x
dc.identifier.citationInternational Journal of Experimental Pathology. Malden: Wiley-blackwell Publishing, Inc, v. 91, n. 2, p. 144-154, 2010.
dc.identifier.doi10.1111/j.1365-2613.2009.00696.x
dc.identifier.issn0959-9673
dc.identifier.lattes1445259468526188
dc.identifier.lattes0947193347312157
dc.identifier.orcid0000-0002-0970-4288
dc.identifier.orcid0000-0002-3622-460X
dc.identifier.urihttp://hdl.handle.net/11449/21600
dc.identifier.wosWOS:000275205700065
dc.language.isoeng
dc.publisherWiley-Blackwell Publishing, Inc
dc.relation.ispartofInternational Journal of Experimental Pathology
dc.relation.ispartofjcr1.938
dc.relation.ispartofsjr0,712
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectandrogen receptoren
dc.subjectApoptosisen
dc.subjectCell proliferationen
dc.subjectDiabetesen
dc.subjectp63en
dc.subjectProstateen
dc.titleProliferation and apoptotic rates and increased frequency of p63-positive cells in the prostate acinar epithelium of alloxan-induced diabetic ratsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-blackwell Publishing, Inc
unesp.author.lattes1445259468526188
unesp.author.lattes0947193347312157[5]
unesp.author.orcid0000-0002-0970-4288[4]
unesp.author.orcid0000-0002-3622-460X[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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