Trypanosoma cruzi: Evaluation of (-)-cubebin derivatives activity in the messenger RNAs processing

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dc.contributor.authorSilva, Márcio Luís Andrade e
dc.contributor.authorCicarelli, Regina Maria Barretto [UNESP]
dc.contributor.authorPauletti, Patrícia M.
dc.contributor.authorLuz, Priscilla Paiva
dc.contributor.authorRezende, Karen Cristina Souza
dc.contributor.authorJanuário, Ana Helena
dc.contributor.authorSilva, Rosangela da
dc.contributor.authorPereira, Ana Carolina
dc.contributor.authorBastos, Jairo Kenupp
dc.contributor.authorAlbuquerque, Sérgio de
dc.contributor.authorMagalhães, Lizandra Guidi
dc.contributor.authorCunha, Wilson Roberto
dc.contributor.institutionUniversidade de Franca (UNIFRAN)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:25:57Z
dc.date.available2014-05-27T11:25:57Z
dc.date.issued2011-08-01
dc.description.abstractNo fully effective treatment has been developed since the discovery of Chagas' disease. Since drug-resistant Trypanosoma cruzi strains are occurring and the current therapy is effective in the acute phase but with various adverse side effects, more studies are needed to characterize the susceptibility of T. cruzi to new drugs. Pre-mRNA maturation in trypanosomatids occurs through a process called trans-splicing, which is unusual RNA processing reaction, and it implies the processing of polycistronic transcription units into individual mRNAs; a short transcript spliced leader (SL RNA) is trans-spliced to the acceptor pre-mRNA, giving origin to the mature mRNA. Cubebin derivatives seem to provide treatments with less collateral effects than benznidazole and showed similar or better trypanocidal activities than benznidazole. Therefore, the cubebin derivatives ((-)-6,6′-dinitrohinokinin (DNH) and (-)-hinokinin (HQ)) interference in the mRNA processing was evaluated using T. cruzi permeable cells (Y and BOL (Bolivia) strains) following by RNase protection reaction. These substances seem to intervene in any step of the RNA transcription, promoting alterations in the RNA synthesis, even though the RNA processing mechanism still occurs. Furthermore, HQ presented better activity against the parasites than DNH, meaning that BOL strain seems to be more resistant than Y. © 2011 Springer-Verlag.en
dc.description.affiliationGrupo de Pesquisas em Produtos Naturais, Nucleo de Pesquisas em Ciencias Exatas e Tecnologicas Universidade de Franca, Franca, São Paulo 14404-600
dc.description.affiliationDepartamento de Ciências Farmacêuticas Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo, Ribeirão Preto, São Paulo 14040-903
dc.description.affiliationDepartamento de Análises Clínicas, Toxicológicas e Bromatológicas Universidade de São Paulo, Ribeirão Preto, São Paulo 14040-903
dc.description.affiliationDepartamento de Ciências Biológicas Faculdade de Ciências Farmacêuticas Universidade Do Estado de São Paulo, Araraquara, São Paulo
dc.format.extent445-451
dc.identifierhttp://dx.doi.org/10.1007/s00436-011-2275-x
dc.identifier.citationParasitology Research, v. 109, n. 2, p. 445-451, 2011.
dc.identifier.doi10.1007/s00436-011-2275-x
dc.identifier.issn0932-0113
dc.identifier.issn1432-1955
dc.identifier.scopus2-s2.0-80052341161
dc.identifier.urihttp://hdl.handle.net/11449/72568
dc.language.isoeng
dc.relation.ispartofParasitology Research
dc.relation.ispartofjcr2.558
dc.relation.ispartofsjr0,991
dc.relation.ispartofsjr0,991
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subject6,6' dinitrohinokinin
dc.subjectbenznidazole
dc.subjectcubebin derivative
dc.subjecthinokinin
dc.subjectmessenger RNA
dc.subjectPiper cubeba extract
dc.subjectplant extract
dc.subjectribonuclease
dc.subjectunclassified drug
dc.subjectanimal cell
dc.subjectantibiotic resistance
dc.subjectantiprotozoal activity
dc.subjectcontrolled study
dc.subjectdrug structure
dc.subjectnonhuman
dc.subjectpiper cubeba
dc.subjectPiperaceae
dc.subjectpriority journal
dc.subjectribonuclease protection assay
dc.subjectRNA processing
dc.subjectRNA synthesis
dc.subjectRNA transcription
dc.subjectstrain difference
dc.subjectTrypanosoma cruzi
dc.subjectAntiprotozoal Agents
dc.subjectLignans
dc.subjectRNA, Messenger
dc.subjectTrans-Splicing
dc.subjectTrypanosomatidae
dc.titleTrypanosoma cruzi: Evaluation of (-)-cubebin derivatives activity in the messenger RNAs processingen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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