Morphology and bacterial colonisation of tooth/ceramic restoration interface after different cement excess removal techniques

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dc.contributor.authorAnami, Lilian Costa [UNESP]
dc.contributor.authorPereira, Cristiane Aparecida [UNESP]
dc.contributor.authorGuerra, Elen [UNESP]
dc.contributor.authorde Assuncao e Souza, Rodrigo Othavio
dc.contributor.authorCardoso Jorge, Antonio Olavo [UNESP]
dc.contributor.authorBottino, Marco Antonio [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal da Paraíba (UFPB)
dc.date.accessioned2014-05-20T14:04:40Z
dc.date.available2014-05-20T14:04:40Z
dc.date.issued2012-09-01
dc.description.abstractObjectives: To evaluate the influence of different protocols for resin cement removal during cementation on biofilm formation.Methods: Twenty-eight ceramic blocks, which were injected under pressure, were placed over enamel blocks obtained from freshly extracted bovine incisors. The ceramic blocks were cemented to the enamel blocks using a dual-cured resin cement and the excess resin was removed according to the experimental group: TS: Teflon spatula; BR: brush; BR+: brush and polishing; SB+: scalpel blade and polishing. After autoclaving, the samples were colonised by incubation in a sucrose broth suspension standardised with Streptococcus mutans in microaerophilic stove. Specimens were quantitatively analysed for bacterial adherence at the adhesive interface using confocal laser scanning microscopy and counting the colony forming units, and qualitatively analysed using SEM. The roughness (Ra/Rz/RSm) was also analysed. Data were analysed by 1-way ANOVA and Tukey's test (5%).Results: The roughness values ranged from 0.96 to 1.69 mu m for Ra (p > 0.05), from 11.59 to 22.80 mu m for Rz (p = 0.02 < 0.05) and from 293.2 to 534.3 mu m for RSm (p = 0.00). Bacterial adhesion varied between 1,974,000 and 2,814,000 CFU/ml (p = 0.00). Biofilm mean thickness ranged from 0.477 and 0.556 mu m (p > 0.05), whilst the biovolume values were between 0.388 and 0.547 mu m(3)/mu m(2) (p = 0.04). Lower values for roughness, bacterial adhesion, biofilm thickness and biovolume were found with BR, whilst TS presented the highest values for most of the parameters. SEM images confirmed the quantitative values.Conclusions: The restoration margin morphology and interface roughness affects bacterial accumulation. The brush technique promoted less bacterial colonisation at the adhesive interface than did the other removal methods.Clinical significance: The brush technique seems to be a good option for removing the excess resin cement after adhesive cementation in clinical practice, as indicated by its better results with lower bacterial colonisation. (C) 2012 Elsevier Ltd. All rights reserved.en
dc.description.affiliationSão Paulo State Univ UNESP SJC, Dept Dent Mat & Prosthodont, São Paulo, Brazil
dc.description.affiliationSão Paulo State Univ UNESP SJC, Dept Oral Biopathol, São Paulo, Brazil
dc.description.affiliationFed Univ Paraiba UFPB, Dept Restorat Dent, Div Prosthodont, Joao Pessoa, Paraiba, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP SJC, Dept Dent Mat & Prosthodont, São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP SJC, Dept Oral Biopathol, São Paulo, Brazil
dc.format.extent742-749
dc.identifierhttp://dx.doi.org/10.1016/j.jdent.2012.05.005
dc.identifier.citationJournal of Dentistry. Oxford: Elsevier B.V., v. 40, n. 9, p. 742-749, 2012.
dc.identifier.doi10.1016/j.jdent.2012.05.005
dc.identifier.issn0300-5712
dc.identifier.lattes0053567153623569
dc.identifier.lattes9234456003563666
dc.identifier.urihttp://hdl.handle.net/11449/22691
dc.identifier.wosWOS:000306973800006
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Dentistry
dc.relation.ispartofjcr3.770
dc.relation.ispartofsjr1,919
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBacterial colonisationen
dc.subjectRemoval techniquesen
dc.subjectRestoration marginen
dc.subjectRoughnessen
dc.subjectToothen
dc.subjectCeramicen
dc.titleMorphology and bacterial colonisation of tooth/ceramic restoration interface after different cement excess removal techniquesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes0053567153623569[5]
unesp.author.lattes9234456003563666
unesp.author.orcid0000-0002-1747-6158[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Ciência e Tecnologia, São José dos Campospt

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Artigos - Materiais Odontológicos e Prótese - ICT
Artigos - Biociências e Diagnóstico Bucal - ICT