TNF alpha contributes for attenuating both Y(397)FAK and Y(416)Src phosphorylations in osteoblasts

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dc.contributor.authorCavagis, A. D. M.
dc.contributor.authorTakamori, E. R.
dc.contributor.authorGranjeiro, J. M.
dc.contributor.authorOliveira, R. C.
dc.contributor.authorFerreira, C. V.
dc.contributor.authorPeppelenbosch, M. P.
dc.contributor.authorZambuzzi, W. F. [UNESP]
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniv Grande Rio UNIGRANRIO
dc.contributor.institutionDiretoria Programas DIPRO Bioengn
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniv Groningen
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-03-18T15:55:20Z
dc.date.available2015-03-18T15:55:20Z
dc.date.issued2014-11-01
dc.description.abstractObjectiveOur poor understanding of how inflammatory mediators can affect osteoblast behavior led us to investigate the tumor necrosis factor (TNF)-induced focal adhesion kinase (FAK) and Src phosphorylation.Material and MethodsMC3T3-E1 pre-osteoblast cells were harvested at specific time points after either TNF treatment or RAW267 stimulated conditioned medium, and thereafter cell extracts were prepared for Immunoblotting assay. ELISA detected TNF content at conditioned medium. Tumor necrosis factor--neutralizing antibodies also were used.ResultsIt was possible to show that TNF provokes attenuation at Y-phosphorylation of both FAK (at Y-397) and Src (at Y-416) proteins (P<0.05), suggesting a decrease in their activities. The very similar profile was observed when osteoblasts were incubated with conditioned medium from lipopolysaccharide (LPS)-stimulated macrophages, it being significantly different than control (FAK and Src, P<0.05). Nevertheless, in order to validate these findings, we decided to pre-incubate osteoblasts with anti-TNF neutralizing antibody (2gml(-1)) prior exposing to conditioned medium. Importantly, our results revealed that there was a diminution on those conditioned medium effects when the same biological parameters were evaluated (P<0.05). Moreover, we also showed that TNF impairs osteoblast adhesion, suggesting an interesting role on osteoblast performance.ConclusionsAltogether, these results suggest that LPS-stimulated macrophage mediators attenuate both FAK and Src activations in osteoblast, suggesting a novel role for TNF on osteoblast performance.en
dc.description.affiliationFed Univ Sao Carlos UFSCar, Sorocaba, *, *
dc.description.affiliationUniv Grande Rio UNIGRANRIO, Escola Ciencias Saude, Lab Multidisciplinar Pesquisa Odontol LAMP, Duque De Caxias, *, *
dc.description.affiliationDiretoria Programas DIPRO Bioengn, Inst Nacl Metrol Normalizacao & Qualidade Ind INM, Xerem, *, *
dc.description.affiliationFOB USP, Bauru Dent Sch, Dept Biol Sci, Bauru, *, *
dc.description.affiliationState Univ Campinas UNICAMP, Dept Biochem, Inst Biol, Campinas, SP, Brazil
dc.description.affiliationUniv Groningen, Univ Med Ctr Groningen, Dept Cell Biol, Kinome Profiling Unit, Groningen, Netherlands
dc.description.affiliationUNESP, Biosci Inst, Dept Chem & Biochem, Lab Bioensaios & Dinam Celular, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Biosci Inst, Dept Chem & Biochem, Lab Bioensaios & Dinam Celular, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent780-786
dc.identifierhttp://dx.doi.org/10.1111/odi.12202
dc.identifier.citationOral Diseases. Hoboken: Wiley-blackwell, v. 20, n. 8, p. 780-786, 2014.
dc.identifier.doi10.1111/odi.12202
dc.identifier.issn1354-523X
dc.identifier.urihttp://hdl.handle.net/11449/117154
dc.identifier.wosWOS:000344395800009
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofOral Diseases
dc.relation.ispartofjcr2.310
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectcell signalingen
dc.subjectfocal adhesion kinaseen
dc.subjectinflammationen
dc.subjectLipopolysaccharideen
dc.subjectmacrophagesen
dc.subjectosteoblastsen
dc.subjectperiodontal diseaseen
dc.subjectSrcen
dc.subjecttumor necrosis factoren
dc.titleTNF alpha contributes for attenuating both Y(397)FAK and Y(416)Src phosphorylations in osteoblastsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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