Investigation into the suitability of screen printed graphene-melanin pH sensors for use in bacterial culturing applications

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dc.contributor.authorWhelan, Simon Peter
dc.contributor.authorTehrani, Zari
dc.contributor.authorPeacock, Martin
dc.contributor.authorPaulin, Joao Vitor [UNESP]
dc.contributor.authorGuy, Owen
dc.contributor.authorGethin, David
dc.contributor.institutionSwansea University
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionZimmer & Peacock AS – Bygg 24
dc.date.accessioned2022-04-28T19:47:49Z
dc.date.available2022-04-28T19:47:49Z
dc.date.issued2022-01-01
dc.description.abstractScreen printed pH sensors have been developed that utilise conductive carbon/graphene coated with DMSO-melanin for pH sensitivity. Their effectiveness has been demonstrated in buffer solutions and here they have been tested in a mixture of various frequently used culturing media solutions along side live bacteria in order to assess their suitability for monitoring the pH in real world bacterial culturing applications. The results indicate that these sensors can be used to accurately and consistently determine the pH of culturing media solutions at a high sensitivity in the presence of bacterial growth over the biologically relevant range of pH5 to pH8. This sensitivity was consistent between different types of culturing media that the sensor was tested with (brain heart infusion, nutrient and lysogeny broth) as well as pH reference buffer solutions. Therefore based on these findings it can be concluded that this sensor is sufficiently robust and has the potential to be useful in applications such as monitoring the ambient conditions of cultured microorganisms in a bioreactor.en
dc.description.affiliationWelsh Centre for Printing and Coating College of Engineering Swansea University
dc.description.affiliationCentre for Nanohealth Swansea University College of Engineering Swansea University
dc.description.affiliationSao Paulo State University (UNESP) Department of Physics School of Sciences
dc.description.affiliationDepartment of Chemistry Swansea University
dc.description.affiliationFuture Manufacturing Research Institute College of Engineering Swansea University
dc.description.affiliationZimmer & Peacock AS – Bygg 24, Nedre vei 8
dc.description.affiliationUnespSao Paulo State University (UNESP) Department of Physics School of Sciences
dc.identifierhttp://dx.doi.org/10.1016/j.jelechem.2021.115868
dc.identifier.citationJournal of Electroanalytical Chemistry, v. 904.
dc.identifier.doi10.1016/j.jelechem.2021.115868
dc.identifier.issn1572-6657
dc.identifier.scopus2-s2.0-85120449800
dc.identifier.urihttp://hdl.handle.net/11449/222971
dc.language.isoeng
dc.relation.ispartofJournal of Electroanalytical Chemistry
dc.sourceScopus
dc.subjectBiosensors
dc.subjectGraphene
dc.subjectMicrobial culture
dc.subjectpH
dc.subjectScreen printed electrode
dc.titleInvestigation into the suitability of screen printed graphene-melanin pH sensors for use in bacterial culturing applicationsen
dc.typeArtigo

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