Expression of bta-miR-222 and LHCGR in bovine cultured granulosa cells: Impact of follicle deviation and regulation by FSH/insulin in vitro

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dc.contributor.authorSantos, P. H. [UNESP]
dc.contributor.authorNunes, S. G. [UNESP]
dc.contributor.authorFranchi, F. F. [UNESP]
dc.contributor.authorGiroto, A. B.
dc.contributor.authorFontes, P. K. [UNESP]
dc.contributor.authorPinheiro, V. G. [UNESP]
dc.contributor.authorCastilho, A. C.S.
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity of Western São Paulo (Unoeste)
dc.date.accessioned2022-04-28T19:50:20Z
dc.date.available2022-04-28T19:50:20Z
dc.date.issued2022-04-01
dc.description.abstractFinal antral follicle development and future ovulation are mediated by gonadotropin-induced changes with spatio-temporally regulated expression of genes. Here, we aimed to quantify the relative mRNA abundance of bta-miR-222 and its predicted target, LHCGR, in granulosa cells (GCs) from follicles, after follicle deviation, as well as from GCs cultured in vitro with follicle stimulating hormone (FSH) and/or insulin. Thus, to study the impact of follicle deviation, Nelore heifers (n = 10; Bos taurus indicus) were hormonally synchronized and slaughtered 3 days after ovulation. Then, GCs from the dominant follicle (DF) and its respective subordinate follicle (SF) were recovered for RT-qPCR. For in vitro analysis, small follicles (2–5 mm) were dissected from bovine ovaries collected from a local abattoir. The GCs were isolated and cultured in serum-free medium, or treated with insulin (1 ng/mL or 10 ng/mL) alone or in combination with human recombinant FSH (1 ng/mL), for 6 days. Our findings showed that the relative mRNA abundance of LHCGR in GCs was higher in the DF compared to the SF (p = 0.01). Inversely, bta-miR-222 expression was lower in the DF compared to the SF (p = 0.01). Furthermore, GCs cultured with FSH and insulin together resulted in a higher abundance of LHCGR and a lower abundance of bta-miR-222 (p ≤ 0.05) when compared to GCs cultured with insulin alone. In conclusion, we found that the LHCGR upregulation in GCs from the DF is inversely related to bta-miR-222 expression. We also suggest the involvement of FSH in bta-miR-222 suppression in healthy bovine GCs.en
dc.description.affiliationSão Paulo State University (Unesp) Department of Pharmacology Institute of Biosciences
dc.description.affiliationUniversity of Western São Paulo (Unoeste)
dc.description.affiliationUnespSão Paulo State University (Unesp) Department of Pharmacology Institute of Biosciences
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 001
dc.description.sponsorshipIdFAPESP: 2013/11480-3
dc.description.sponsorshipIdFAPESP: 2015/04505-5
dc.format.extent71-77
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2022.01.034
dc.identifier.citationTheriogenology, v. 182, p. 71-77.
dc.identifier.doi10.1016/j.theriogenology.2022.01.034
dc.identifier.issn0093-691X
dc.identifier.scopus2-s2.0-85123953555
dc.identifier.urihttp://hdl.handle.net/11449/223396
dc.language.isoeng
dc.relation.ispartofTheriogenology
dc.sourceScopus
dc.subjectAntral follicle
dc.subjectCattle
dc.subjectFollicle divergence
dc.subjectGene expression
dc.subjectLHCGR
dc.subjectMicroRNA
dc.titleExpression of bta-miR-222 and LHCGR in bovine cultured granulosa cells: Impact of follicle deviation and regulation by FSH/insulin in vitroen
dc.typeArtigo
unesp.author.orcid0000-0003-1666-7021[7]

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