Aspectos fisiológicos, celulares e moleculares da tolerância à dessecação em sementes de Anadenanthera colubrina durante a germinação

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dc.contributor.authorCastro, L. E. [UNESP]
dc.contributor.authorGuimarães, C. C.
dc.contributor.authorFaria, J. M.R.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Lavras (UFLA)
dc.date.accessioned2018-12-11T16:49:53Z
dc.date.available2018-12-11T16:49:53Z
dc.date.issued2017-11-01
dc.description.abstractDuring germination, orthodox seeds become gradually intolerant to desiccation, and for this reason, they are a good model for recalcitrance studies. In the present work, physiological, biochemical, and ultrastructural aspects of the desiccation tolerance were characterized during the germination process of Anadenanthera colubrina seeds. The seeds were imbibed during zero (control), 2, 8, 12 (no germinated seeds), and 18 hours (germinated seeds with 1 mm protruded radicle); then they were dried for 72 hours, rehydrated and evaluated for survivorship. Along the imbibition, cytometric and ultrastructural analysis were performed, besides the extraction of the heat-stable proteins. Posteriorly to imbibition and drying, the evaluation of ultrastructural damages was performed. Desiccation tolerance was fully lost after root protrusion. There was no increase in 4C DNA content after the loss of desiccation tolerance. Ultrastructural characteristics of cells from 1mm roots resembled those found in the recalcitrant seeds, in both hydrated and dehydrated states. The loss of desiccation tolerance coincided with the reduction of heat-stable proteins.en
dc.description.affiliationInstituto de Biociências Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESP, Avenida 24-A, 1515, Bairro Bela Vista
dc.description.affiliationDepartment of Agriculture Universidade Federal de Lavras – UFLA, CP 3037
dc.description.affiliationLaboratory of Tree Seeds Department of Forest Sciences Universidade Federal de Lavras – UFLA, CP 3037
dc.description.affiliationUnespInstituto de Biociências Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESP, Avenida 24-A, 1515, Bairro Bela Vista
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent774-780
dc.identifierhttp://dx.doi.org/10.1590/1519-6984.00616
dc.identifier.citationBrazilian Journal of Biology, v. 77, n. 4, p. 774-780, 2017.
dc.identifier.doi10.1590/1519-6984.00616
dc.identifier.fileS1519-69842017000400774.pdf
dc.identifier.issn1678-4375
dc.identifier.issn1519-6984
dc.identifier.scieloS1519-69842017000400774
dc.identifier.scopus2-s2.0-85030684616
dc.identifier.urihttp://hdl.handle.net/11449/170236
dc.language.isoeng
dc.relation.ispartofBrazilian Journal of Biology
dc.relation.ispartofsjr0,523
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectDesiccation tolerance
dc.subjectDrying
dc.subjectForest seeds
dc.titleAspectos fisiológicos, celulares e moleculares da tolerância à dessecação em sementes de Anadenanthera colubrina durante a germinaçãopt
dc.title.alternativePhysiological, cellular and molecular aspects of the desiccation tolerance in Anadenanthera colubrina seeds during germinationen
dc.typeArtigo

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