Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom

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dc.contributor.authorUllah, Anwar [UNESP]
dc.contributor.authorde Giuseppe, Priscila Oliveira
dc.contributor.authorMurakami, Mario Tyago
dc.contributor.authorTrevisan-Silva, Dilza
dc.contributor.authorMartins Wille, Ana Carolina
dc.contributor.authorChaves-Moreira, Daniele
dc.contributor.authorGremski, Luiza Helena
dc.contributor.authorda Silveira, Rafael Bertoni
dc.contributor.authorSennf-Ribeiro, Andrea
dc.contributor.authorChaim, Olga Meiri
dc.contributor.authorVeiga, Silvio Sanches
dc.contributor.authorArni, Raghuvir Krishnaswamy [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionCtr Nacl Pesquisa Energia & Mat
dc.contributor.institutionUniversidade Federal do Paraná (UFPR)
dc.contributor.institutionUniv Estadual Ponta Grossa
dc.date.accessioned2014-05-20T14:02:41Z
dc.date.available2014-05-20T14:02:41Z
dc.date.issued2011-02-01
dc.description.abstractPhospholipases D are the major dermonecrotic component of Loxosceles venom and catalyze the hydrolysis of phospholipids, resulting in the formation of lipid mediators such as ceramide-1-phosphate and lysophosphatidic acid which can induce pathological and biological responses. Phospholipases D can be classified into two classes depending on their catalytic efficiency and the presence of an additional disulfide bridge. In this work, both wild-type and H12A-mutant forms of the class II phospholipase D from L. intermedia venom were crystallized. Wild-type and H12A-mutant crystals were grown under very similar conditions using PEG 200 as a precipitant and belonged to space group P12(1)1, with unit-cell parameters a = 50.1, b = 49.5, c = 56.5 angstrom, beta = 105.9 degrees. Wild-type and H12A-mutant crystals diffracted to maximum resolutions of 1.95 and 1.60 angstrom, respectively.en
dc.description.affiliationUniv Estadual Paulista, UNESP, Dept Fis, Ctr Multiusuario Inovacao Biomol, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationCtr Nacl Pesquisa Energia & Mat, LNBio, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv Fed Parana, Dept Biol Celular, BR-81531960 Curitiba, Parana, Brazil
dc.description.affiliationUniv Estadual Ponta Grossa, Dept Biol Estrutural Biol Mol & Genet, BR-84030900 Ponta Grossa, PR, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Dept Fis, Ctr Multiusuario Inovacao Biomol, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.sponsorshipSecretaria de Estado de Ciencia, Tecnologia e Ensino Superior (SETI) do Parana
dc.description.sponsorshipFundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Paraná (FAADCT/PR)
dc.description.sponsorshipTWAS
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent234-236
dc.identifierhttp://dx.doi.org/10.1107/S1744309110050931
dc.identifier.citationActa Crystallographica Section F-structural Biology and Crystallization Communications. Malden: Wiley-blackwell, v. 67, p. 234-236, 2011.
dc.identifier.doi10.1107/S1744309110050931
dc.identifier.fileWOS000287030600013.pdf
dc.identifier.issn1744-3091
dc.identifier.lattes9162508978945887
dc.identifier.orcid0000-0003-2460-1145
dc.identifier.urihttp://hdl.handle.net/11449/22098
dc.identifier.wosWOS:000287030600013
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofActa Crystallographica Section F: Structural Biology and Crystallization Communications
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleCrystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venomen
dc.typeArtigo
dcterms.licensehttp://journals.iucr.org/services/copyrightpolicy.html
dcterms.rightsHolderWiley-blackwell
unesp.author.lattes9162508978945887[12]
unesp.author.orcid0000-0003-2460-1145[12]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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Artigos - Física - IBILCE