Assessing the usefulness of B-mode and colour Doppler sonography, and measurements of circulating progesterone concentrations for determining ovarian responses in superovulated ewes

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dc.contributor.authorOliveira, M. E. F. [UNESP]
dc.contributor.authorRibeiro, I. F. [UNESP]
dc.contributor.authorRodriguez, M. G. K. [UNESP]
dc.contributor.authorMaciel, G. S. [UNESP]
dc.contributor.authorFonseca, J. F.
dc.contributor.authorBrandao, F. Z.
dc.contributor.authorBartlewski, P. M.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionFluminense Fed Univ
dc.contributor.institutionUniv Guelph
dc.date.accessioned2018-11-26T17:51:35Z
dc.date.available2018-11-26T17:51:35Z
dc.date.issued2018-06-01
dc.description.abstractContents The main goal of this study was to assess the usefulness of two imaging modalities, namely the B-mode and colour Doppler sonography, and serum progesterone (P-4) concentrations for determining the ovarian response in superovulated ewes. Twenty-four sexually mature Santa Ines ewes underwent the superovulatory treatment consisting of eight injections of porcine FSH (total dose of 200 or 133 or 100mg; n=8 ewes/total dose) given at 12-hr intervals and initiated 48hr before CIDR (R) (Pfizer Inc., Auckland, New Zealand) removal. Six days after natural mating, the ovaries of all donor ewes were visualized and examined with transrectal ultrasonography and then with videolaparoscopy to identify and enumerate corpora lutea (CL) and luteinized unovulated follicles (LUFs). Jugular blood samples were collected just prior to ovarian examinations. The total number of CL (r=.78 and 0.83, p<.0001) and LUFs (r=.74 and 0.90, p<.0001) enumerated using the B-mode and colour Doppler ultrasonographic technique, respectively, were correlated with that ascertained by videolaparoscopy. Circulating concentrations of P-4 were related directly to the number of healthy CL (r=.73, p=.0002) and inversely to the number of prematurely regressing CL (r=-.46, p=.03), but the accuracy of predicting the number of short-lived CL with serum P-4 concentrations was very poor. The present results indicate that ultrasonographic imaging and serum P-4 measurements on the day of embryo recovery are useful indicators of total/normal CL numbers and both ultrasonographic techniques can be used to quantify LUFs in superovulated ewes.en
dc.description.affiliationSao Paulo State Univ, Sch Agr & Vet Sci, Dept Preventat Vet Med & Anim Reprod, Jaboticabal, Brazil
dc.description.affiliationBrazilian Agr Res Corp Embrapa Goats & Sheep, Coronel Pacheco, Brazil
dc.description.affiliationFluminense Fed Univ, Fac Vet Med, Niteroi, RJ, Brazil
dc.description.affiliationUniv Guelph, Ontario Vet Coll, Dept Biomed Sci, Guelph, ON, Canada
dc.description.affiliationUnespSao Paulo State Univ, Sch Agr & Vet Sci, Dept Preventat Vet Med & Anim Reprod, Jaboticabal, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipBrazilian Agricultural Research Corporation
dc.description.sponsorshipPROPE-Unesp
dc.description.sponsorshipIdFAPESP: 2015/13350-5
dc.description.sponsorshipIdFAPESP: 2014/04614-6
dc.description.sponsorshipIdBrazilian Agricultural Research Corporation: 02.13.06.026.00.03
dc.description.sponsorshipIdPROPE-Unesp: TC1288/2015
dc.format.extent742-750
dc.identifierhttp://dx.doi.org/10.1111/rda.13165
dc.identifier.citationReproduction In Domestic Animals. Hoboken: Wiley, v. 53, n. 3, p. 742-750, 2018.
dc.identifier.doi10.1111/rda.13165
dc.identifier.issn0936-6768
dc.identifier.urihttp://hdl.handle.net/11449/164179
dc.identifier.wosWOS:000432030900020
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofReproduction In Domestic Animals
dc.relation.ispartofsjr0,594
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectlaparoscopy
dc.subjectluteal structures
dc.subjectovary
dc.subjectprogesterone
dc.subjectSanta Ines ewes
dc.subjectsuperovulation
dc.subjectultrasonography
dc.titleAssessing the usefulness of B-mode and colour Doppler sonography, and measurements of circulating progesterone concentrations for determining ovarian responses in superovulated ewesen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell

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Artigos - Medicina Veterinária Preventiva e Reprodução Animal - FCAV