Phospholipase A1-based cross-reactivity among venoms of clinically relevant Hymenoptera from Neotropical and temperate regions

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dc.contributor.authorPerez-Riverol, Amilcar [UNESP]
dc.contributor.authorFernandes, Luís Gustavo Romani
dc.contributor.authorMusacchio Lasa, Alexis
dc.contributor.authordos Santos-Pinto, José Roberto Aparecido [UNESP]
dc.contributor.authorMoitinho Abram, Débora
dc.contributor.authorIzuka Moraes, Gabriel Hideki [UNESP]
dc.contributor.authorJabs, Frederic
dc.contributor.authorMiehe, Michaela
dc.contributor.authorSeismman, Henning
dc.contributor.authorPalma, Mario Sergio [UNESP]
dc.contributor.authorde Lima Zollner, Ricardo
dc.contributor.authorSpillner, Edzard
dc.contributor.authorBrochetto-Braga, Márcia Regina [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionSystem Biology Department
dc.contributor.institutionAarhus University
dc.contributor.institutionEuroimmun AG
dc.date.accessioned2018-12-11T17:16:09Z
dc.date.available2018-12-11T17:16:09Z
dc.date.issued2018-01-01
dc.description.abstractMolecular cross-reactivity caused by allergen homology or cross-reactive carbohydrate determinants (CCDs) is a major challenge for diagnosis and immunotherapy of insect venom allergy. Venom phospholipases A1 (PLA1s) are classical, mostly non-glycosylated wasp and ant allergens that provide diagnostic benefit for differentiation of genuine sensitizations from cross-reactivity. As CCD-free molecules, venom PLA1s are not causative for CCD-based cross-reactivity. Little is known however about the protein-based cross-reactivity of PLA1 within vespid species. Here, we address PLA1-based cross-reactivity among ten clinically relevant Hymenoptera venoms from Neotropical and temperate regions including Polybia paulista (paulistinha) venom and Vespula vulgaris (yellow jacket) venom. In order to evaluate cross-reactivity, sera of mice sensitized with recombinant PLA1 (rPoly p 1) from P. paulista wasp venom were used. Pronounced IgE and IgG based cross-reactivity was detected for wasp venoms regardless the geographical region of origin. The cross-reactivity correlated well with the identity of the primary sequence and 3-D models of PLA1 proteins. In contrast, these mice sera showed no reaction with honeybee (HBV) and fire ant venom. Furthermore, sera from patients monosensitized to HBV and fire ants did not recognize the rPoly p 1 in immunoblotting. Our findings reveal the presence of conserved epitopes in the PLA1s from several clinically relevant wasps as major cause of PLA1-based in vitro cross-reactivity. These findings emphasize the limitations but also the potential of PLA1-based HVA diagnostics.en
dc.description.affiliationLaboratório de Biologia Molecular de Artrópodes-LBMA-IBRC-UNESP (Univ Estadual Paulista), Av. 24-A, n_1515, Bela Vista
dc.description.affiliationLaboratório de Imunologia Translacional Faculdade de Ciências Médicas FCM Universidade Estadual de Campinas-UNICAMP, Rua Vital Brasil, n_ 300, Cidade Universitária “Zeferino Vaz”
dc.description.affiliationCenter for Genetic Engineering and Biotechnology Biomedical Research Division System Biology Department, Ave. 31, e/158 and 190, P.O. Box 6162, Cubanacan, Playa
dc.description.affiliationCentro de Estudos de Insetos Sociais-CEIS-IBRC-UNESP (Univ Estadual Paulista), Av. 24-A, n° 1515, Bela Vista
dc.description.affiliationImmunological Engineering Department of Engineering Aarhus University, Gustav Wieds Vej 10
dc.description.affiliationEuroimmun AG, Seekamp 21
dc.description.affiliationCentro de Estudos de Venenos e Animais Peçonhentos-CEVAP (Univ Estadual Paulista), Rua José Barbosa de Barros, 1780, Fazenda Experimental Lageado
dc.description.affiliationUnespLaboratório de Biologia Molecular de Artrópodes-LBMA-IBRC-UNESP (Univ Estadual Paulista), Av. 24-A, n_1515, Bela Vista
dc.description.affiliationUnespCentro de Estudos de Insetos Sociais-CEIS-IBRC-UNESP (Univ Estadual Paulista), Av. 24-A, n° 1515, Bela Vista
dc.description.affiliationUnespCentro de Estudos de Venenos e Animais Peçonhentos-CEVAP (Univ Estadual Paulista), Rua José Barbosa de Barros, 1780, Fazenda Experimental Lageado
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2013/26451-9
dc.format.extent87-93
dc.identifierhttp://dx.doi.org/10.1016/j.molimm.2017.11.007
dc.identifier.citationMolecular Immunology, v. 93, p. 87-93.
dc.identifier.doi10.1016/j.molimm.2017.11.007
dc.identifier.file2-s2.0-85034665201.pdf
dc.identifier.issn1872-9142
dc.identifier.issn0161-5890
dc.identifier.lattes2901888624506535
dc.identifier.scopus2-s2.0-85034665201
dc.identifier.urihttp://hdl.handle.net/11449/175522
dc.language.isoeng
dc.relation.ispartofMolecular Immunology
dc.relation.ispartofsjr1,352
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCross-reactivity
dc.subjectInsect venom allergy
dc.subjectMolecular diagnosis
dc.subjectPhospholipase A1
dc.subjectSensitization
dc.titlePhospholipase A1-based cross-reactivity among venoms of clinically relevant Hymenoptera from Neotropical and temperate regionsen
dc.typeArtigo
unesp.author.lattes2901888624506535

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Artigos - Biologia - IBRC
Botucatu - CEVAP - Centro de Estudos de Venenos e Animais Peçonhentos
Rio Claro - CEIS - Centro de Estudos de Insetos Sociais