Inhibitory effect of gallic acid and its esters on 2,2'-azobis(2-amidinopropane)hodrpchloride (AAPH)-induced hemolysis and depletion of intracellular glutathione in erythrocytes

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dc.contributor.authorXimenes, Valdecir Farias [UNESP]
dc.contributor.authorLopes, Mariana G. [UNESP]
dc.contributor.authorPetrônio, Maicon Segalla [UNESP]
dc.contributor.authorRegasini, Luis Octavio [UNESP]
dc.contributor.authorSilva, Dulce Helena Siqueira [UNESP]
dc.contributor.authorFonseca, Luiz Marcos da [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2016-01-28T16:56:18Z
dc.date.available2016-01-28T16:56:18Z
dc.date.issued2010
dc.description.abstractThe protective effect of gallic acid and its esters, methyl, propyl, and lauryl gallate, against 2,2'-azobis(2-amidinopropane)hydrochloride (AAPH)-induced hemolysis and depletion of intracellular glutathione (GSH) in erythrocytes was studied. The inhibition of hemolysis was dose-dependent, and the esters were significantly more effective than gallic acid. Gallic acid and its esters were compared with regard to their reactivity to free radicals, using the DPPH and AAPH/pyranine free-cell assays, and no significant difference was obtained. Gallic acid and its esters not only failed to inhibit the depletion of intracellular GSH in erythrocytes induced by AAPH but exacerbated it. Similarly, the oxidation of GSH by AAPH or horseradish peroxidase/H(2)O(2) in cell-free systems was exacerbated by gallic acid or gallates. This property could be involved in the recent findings on pro-apoptotic and pro-oxidant activities of gallates in tumor cells. We provide evidence that lipophilicity and not only radical scavenger potency is an important factor regarding the efficiency of antihemolytic substances.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas de Araraquara
dc.description.affiliationDepartamento de Química, Faculdade de Ciências, Unesp − Univ Estadual Paulista, Bauru, São Paulo (SP), CEP 17033-360, Brazil
dc.description.affiliationDepartamento de Química Orgânica, Instituto de Química, Unesp − Univ Estadual Paulista, Araraquara, São Paulo (SP), Brazil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas de Araraquara
dc.description.affiliationUnespDepartamento de Química, Faculdade de Ciências, Unesp − Univ Estadual Paulista, Bauru, São Paulo (SP), CEP 17033-360, Brazil
dc.description.affiliationUnespDepartamento de Química Orgânica, Instituto de Química, Unesp − Univ Estadual Paulista, Araraquara, São Paulo (SP), Brazil
dc.format.extent5355-5362
dc.identifierhttp://pubs.acs.org/doi/abs/10.1021/jf100233y
dc.identifier.citationJournal of Agricultural and Food Chemistry, v. 58, p. 5355-5362, 2010.
dc.identifier.doi10.1021/jf100233y
dc.identifier.issn0021-8561
dc.identifier.lattes4419635633356792
dc.identifier.lattes0992736452764550
dc.identifier.lattes4702004904231248
dc.identifier.lattes4066413997908572
dc.identifier.orcid0000-0002-1516-7765
dc.identifier.urihttp://hdl.handle.net/11449/133717
dc.language.isoeng
dc.relation.ispartofJournal of Agricultural and Food Chemistry
dc.relation.ispartofjcr3.412
dc.relation.ispartofsjr1,269
dc.rights.accessRightsAcesso restrito
dc.sourceCurrículo Lattes
dc.subjectGallic aciden
dc.subjectGallatesen
dc.subjectErythrocytesen
dc.subjectHemolysisen
dc.subjectGlutathioneen
dc.subjectPeroxyl radicalen
dc.subjectAntioxidanten
dc.subjectPro-oxidant activityen
dc.titleInhibitory effect of gallic acid and its esters on 2,2'-azobis(2-amidinopropane)hodrpchloride (AAPH)-induced hemolysis and depletion of intracellular glutathione in erythrocytesen
dc.typeArtigo
unesp.author.lattes4419635633356792
unesp.author.lattes0992736452764550
unesp.author.lattes4702004904231248[5]
unesp.author.lattes4066413997908572
unesp.author.orcid0000-0002-1516-7765[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.departmentAnálises Clínicaspt
unesp.departmentQuímica - FCpt

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Artigos - Análises Clínicas - FCFAR
Artigos - Química Orgânica - IQ
Bauru - FC - Faculdade de Ciências